Self-crosslinked polyethylenimine nanogels for enhanced intracellular delivery of siRNA

Chunsoo Kim; Yuhan Lee; Soo Hyeon Lee; Jee Seon Kim; Ji Hoon Jeong; Tae Gwan Park

doi:10.1007/s13233-011-0207-3

Self-crosslinked polyethylenimine nanogels for enhanced intracellular delivery of siRNA

Chunsoo Kim, Yuhan Lee, Soo Hyeon Lee, Jee Seon Kim, Ji Hoon Jeong, Tae Gwan Park

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Branched polyethylenimine (bPEI) (M _w =25 k) conjugated with catechol groups (PEI-C) was crosslinked to produce PEI-catechol nanogels (PCNs), which were used as a new class of small interfering RNA (siRNA) delivery carriers. The conjugated catechol groups were crosslinked with the primary amines of PEI under basic conditions. The size and morphology of the PCNs were analyzed by dynamic light scattering (DLS) and atomic force microscopy (AFM). The resulting PCNs were quite stable and had an average diameter of 111.4 ± 14.8 nm in an aqueous solution. Compared to bPEI, the PCNs exhibited much lower cytotoxicity, and formed more stable complexes with siRNA. The PCNs/siRNA complexes exhibited enhanced cellular uptake and promoted gene silencing efficiency, suggesting that they can be used potentially as a less cytotoxic siRNA carrier.

Original language	English
Pages (from-to)	166-171
Number of pages	6
Journal	Macromolecular Research
Volume	19
Issue number	2
DOIs	https://doi.org/10.1007/s13233-011-0207-3
State	Published - Feb 2011
Externally published	Yes

Keywords

bio-inspired
catechol
nanogel
polyethylenimine
siRNA

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10.1007/s13233-011-0207-3

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title = "Self-crosslinked polyethylenimine nanogels for enhanced intracellular delivery of siRNA",

abstract = "Branched polyethylenimine (bPEI) (M w =25 k) conjugated with catechol groups (PEI-C) was crosslinked to produce PEI-catechol nanogels (PCNs), which were used as a new class of small interfering RNA (siRNA) delivery carriers. The conjugated catechol groups were crosslinked with the primary amines of PEI under basic conditions. The size and morphology of the PCNs were analyzed by dynamic light scattering (DLS) and atomic force microscopy (AFM). The resulting PCNs were quite stable and had an average diameter of 111.4 ± 14.8 nm in an aqueous solution. Compared to bPEI, the PCNs exhibited much lower cytotoxicity, and formed more stable complexes with siRNA. The PCNs/siRNA complexes exhibited enhanced cellular uptake and promoted gene silencing efficiency, suggesting that they can be used potentially as a less cytotoxic siRNA carrier.",

keywords = "bio-inspired, catechol, nanogel, polyethylenimine, siRNA",

author = "Chunsoo Kim and Yuhan Lee and Lee, {Soo Hyeon} and Kim, {Jee Seon} and Jeong, {Ji Hoon} and Park, {Tae Gwan}",

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T1 - Self-crosslinked polyethylenimine nanogels for enhanced intracellular delivery of siRNA

AU - Kim, Chunsoo

AU - Lee, Yuhan

AU - Lee, Soo Hyeon

AU - Kim, Jee Seon

AU - Jeong, Ji Hoon

AU - Park, Tae Gwan

PY - 2011/2

Y1 - 2011/2

N2 - Branched polyethylenimine (bPEI) (M w =25 k) conjugated with catechol groups (PEI-C) was crosslinked to produce PEI-catechol nanogels (PCNs), which were used as a new class of small interfering RNA (siRNA) delivery carriers. The conjugated catechol groups were crosslinked with the primary amines of PEI under basic conditions. The size and morphology of the PCNs were analyzed by dynamic light scattering (DLS) and atomic force microscopy (AFM). The resulting PCNs were quite stable and had an average diameter of 111.4 ± 14.8 nm in an aqueous solution. Compared to bPEI, the PCNs exhibited much lower cytotoxicity, and formed more stable complexes with siRNA. The PCNs/siRNA complexes exhibited enhanced cellular uptake and promoted gene silencing efficiency, suggesting that they can be used potentially as a less cytotoxic siRNA carrier.

AB - Branched polyethylenimine (bPEI) (M w =25 k) conjugated with catechol groups (PEI-C) was crosslinked to produce PEI-catechol nanogels (PCNs), which were used as a new class of small interfering RNA (siRNA) delivery carriers. The conjugated catechol groups were crosslinked with the primary amines of PEI under basic conditions. The size and morphology of the PCNs were analyzed by dynamic light scattering (DLS) and atomic force microscopy (AFM). The resulting PCNs were quite stable and had an average diameter of 111.4 ± 14.8 nm in an aqueous solution. Compared to bPEI, the PCNs exhibited much lower cytotoxicity, and formed more stable complexes with siRNA. The PCNs/siRNA complexes exhibited enhanced cellular uptake and promoted gene silencing efficiency, suggesting that they can be used potentially as a less cytotoxic siRNA carrier.

KW - bio-inspired

KW - catechol

KW - nanogel

KW - polyethylenimine

KW - siRNA

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JO - Macromolecular Research

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Self-crosslinked polyethylenimine nanogels for enhanced intracellular delivery of siRNA

Abstract

Keywords

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