SEBINI-CABIN: An analysis pipeline for biological network inference, with a case study in protein-protein interaction network reconstruction

The Software Environment for Biological Network Inference (SEBINI) has been created to provide an Interactive environment for the deployment and testing of network inference algorithms that use high-throughput expression data. Networks inferred from the SEBINI software platform can be further analyzed using the Collective Analysis of Biological Interaction Networks (CABIN), software that allows integration and analysis of proteinprotein interaction and gene-to-gene regulatory evidence obtained from multiple sources. In this paper, we present a case study on the SEBINI and CABIN tools for protein-protein interaction network reconstruction. Incorporating the Bayesian Estimator of Protein-Protein Association Probabilities (BEPro) algorithm into the SEBINI toolkit, we have created a pipeline for structural inference and supplemental analysis of proteinprotein interaction networks from sets of mass spectrometry bait-prey experiment data. Demo site for SEBINI: https://www.emsl.pnl.gov /SEBINI/ Contact: [email protected] The BEPro algorithm is available at http://www.pnl.gov/statistics/BEPro3/index.htm. Contact: [email protected]. CABIN can be downloaded from www.sysbio.org/dataresources/ ca.bin.stm. Contact: [email protected].