Screening of potentially hormonally active chemicals using bioluminescent yeast bioreporters

John Sanseverino, Melanie L. Eldridge, Alice C. Layton, James P. Easter, Jason Yarbrough, Terry Wayne Schultz, Gary S. Sayler

Research output: Contribution to journalArticlepeer-review

74 Scopus citations

Abstract

Saccharomyces cerevisiae bioluminescent bioreporter assays were developed previously to assess a chemical's estrogenic or androgenic disrupting potential. S. cerevisiae BLYES, S. cerevisiae BLYAS, S. cerevisiae BLYR, were used to assess their reproducibility and utility in screening 68, 69, and 71 chemicals for estrogenic, androgenic, and toxic effects, respectively. EC50 values were 6.3 ± 2.4 × 10-10M (n = 18) and 1.1 ± 0.5 × 10-8M (n = 13) for BLYES and BLYAS, using 17β-estradiol and 5α-dihydrotestosterone over concentration ranges of 2.5 × 10-12 through 1.0 × 10-6M, respectively. Based on analysis of replicate standard curves and comparison to background controls, a set of quantitative rules have been formulated to interpret data and determine if a chemical is potentially hormonally active, toxic, both, or neither. The results demonstrated that these assays are applicable for Tier I chemical screening in Environmental Protection Agency's Endocrine Disruptor Screening and Testing Program as well as for monitoring endocrine-disrupting activity of unknown chemicals in water.

Original languageEnglish
Pages (from-to)122-134
Number of pages13
JournalToxicological Sciences
Volume107
Issue number1
DOIs
StatePublished - 2009
Externally publishedYes

Keywords

  • Androgens
  • Bioluminescence
  • Biosensing
  • Estrogens
  • Saccharomyces cerevisiae

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