TY - JOUR
T1 - Screening of potentially hormonally active chemicals using bioluminescent yeast bioreporters
AU - Sanseverino, John
AU - Eldridge, Melanie L.
AU - Layton, Alice C.
AU - Easter, James P.
AU - Yarbrough, Jason
AU - Schultz, Terry Wayne
AU - Sayler, Gary S.
PY - 2009
Y1 - 2009
N2 - Saccharomyces cerevisiae bioluminescent bioreporter assays were developed previously to assess a chemical's estrogenic or androgenic disrupting potential. S. cerevisiae BLYES, S. cerevisiae BLYAS, S. cerevisiae BLYR, were used to assess their reproducibility and utility in screening 68, 69, and 71 chemicals for estrogenic, androgenic, and toxic effects, respectively. EC50 values were 6.3 ± 2.4 × 10-10M (n = 18) and 1.1 ± 0.5 × 10-8M (n = 13) for BLYES and BLYAS, using 17β-estradiol and 5α-dihydrotestosterone over concentration ranges of 2.5 × 10-12 through 1.0 × 10-6M, respectively. Based on analysis of replicate standard curves and comparison to background controls, a set of quantitative rules have been formulated to interpret data and determine if a chemical is potentially hormonally active, toxic, both, or neither. The results demonstrated that these assays are applicable for Tier I chemical screening in Environmental Protection Agency's Endocrine Disruptor Screening and Testing Program as well as for monitoring endocrine-disrupting activity of unknown chemicals in water.
AB - Saccharomyces cerevisiae bioluminescent bioreporter assays were developed previously to assess a chemical's estrogenic or androgenic disrupting potential. S. cerevisiae BLYES, S. cerevisiae BLYAS, S. cerevisiae BLYR, were used to assess their reproducibility and utility in screening 68, 69, and 71 chemicals for estrogenic, androgenic, and toxic effects, respectively. EC50 values were 6.3 ± 2.4 × 10-10M (n = 18) and 1.1 ± 0.5 × 10-8M (n = 13) for BLYES and BLYAS, using 17β-estradiol and 5α-dihydrotestosterone over concentration ranges of 2.5 × 10-12 through 1.0 × 10-6M, respectively. Based on analysis of replicate standard curves and comparison to background controls, a set of quantitative rules have been formulated to interpret data and determine if a chemical is potentially hormonally active, toxic, both, or neither. The results demonstrated that these assays are applicable for Tier I chemical screening in Environmental Protection Agency's Endocrine Disruptor Screening and Testing Program as well as for monitoring endocrine-disrupting activity of unknown chemicals in water.
KW - Androgens
KW - Bioluminescence
KW - Biosensing
KW - Estrogens
KW - Saccharomyces cerevisiae
UR - http://www.scopus.com/inward/record.url?scp=58049211932&partnerID=8YFLogxK
U2 - 10.1093/toxsci/kfn229
DO - 10.1093/toxsci/kfn229
M3 - Article
C2 - 18996888
AN - SCOPUS:58049211932
SN - 1096-6080
VL - 107
SP - 122
EP - 134
JO - Toxicological Sciences
JF - Toxicological Sciences
IS - 1
ER -