Salicylate and catechol levels are maintained in nahG transgenic poplar

Alison M. Morse; Timothy J. Tschaplinski; Christopher Dervinis; Paula M. Pijut; Eric A. Schmelz; Wendy Day; John M. Davis

doi:10.1016/j.phytochem.2007.05.014

Salicylate and catechol levels are maintained in nahG transgenic poplar

Alison M. Morse, Timothy J. Tschaplinski, Christopher Dervinis, Paula M. Pijut, Eric A. Schmelz, Wendy Day, John M. Davis

Biosciences Division

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Metabolic profiling was used to investigate the molecular phenotypes of a transgenic Populus tremula × P. alba hybrid expressing the nahG transgene, a bacterial gene encoding salicylate hydroxylase that converts salicylic acid to catechol. Despite the efficacy of this transgenic approach to reduce salicylic acid levels in other model systems and thereby elucidate roles for salicylic acid in plant signaling, transgenic poplars had similar foliar levels of salicylic acid and catechol to that of non-transformed controls and exhibited no morphological phenotypes. To gain a deeper understanding of the basis for these observations, we analyzed metabolic profiles of leaves as influenced by transgene expression. Expression of nahG decreased quinic acid conjugates and increased catechol glucoside, while exerting little effect on levels of salicylic acid and catechol, the substrate and product, respectively, of the nahG enzyme. This suggests a biological role of elevated constitutive salicylic acid levels in Populus, in contrast to other plant systems in which nahG dramatically reduces salicylic acid levels.

Original language	English
Pages (from-to)	2043-2052
Number of pages	10
Journal	Phytochemistry
Volume	68
Issue number	15
DOIs	https://doi.org/10.1016/j.phytochem.2007.05.014
State	Published - Aug 2007

Funding

The work was supported by Department of Energy award DE-FG-02-04ER63845 and Consortium for Plant Biotechnology Award EPA82947901-128 (to J.D.) and, in part, by the Office of Science, Biological, and Environmental Research, US Department of Energy (to T.J.T.). Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for the US Department of Energy under contract DE-AC05-00OR22725. We sincerely thank Katherine Smith for help with plant maintenance and harvesting; Dudley Huber and Tania Quesada for assistance with statistical analyses; and Timothy Martin for light measurements, chlorophyll fluorescence measurements, and helpful discussions. We gratefully acknowledge Syngenta Biotechnology Inc. for their gift of the nahG construct.

Keywords

Catechol
Phenolic glycoside
Populus tremula × P. alba hybrid
Quinic acid
Salicaceae
Salicylate hydroxylase
Salicylic acid
nahG

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10.1016/j.phytochem.2007.05.014

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title = "Salicylate and catechol levels are maintained in nahG transgenic poplar",

abstract = "Metabolic profiling was used to investigate the molecular phenotypes of a transgenic Populus tremula × P. alba hybrid expressing the nahG transgene, a bacterial gene encoding salicylate hydroxylase that converts salicylic acid to catechol. Despite the efficacy of this transgenic approach to reduce salicylic acid levels in other model systems and thereby elucidate roles for salicylic acid in plant signaling, transgenic poplars had similar foliar levels of salicylic acid and catechol to that of non-transformed controls and exhibited no morphological phenotypes. To gain a deeper understanding of the basis for these observations, we analyzed metabolic profiles of leaves as influenced by transgene expression. Expression of nahG decreased quinic acid conjugates and increased catechol glucoside, while exerting little effect on levels of salicylic acid and catechol, the substrate and product, respectively, of the nahG enzyme. This suggests a biological role of elevated constitutive salicylic acid levels in Populus, in contrast to other plant systems in which nahG dramatically reduces salicylic acid levels.",

keywords = "Catechol, Phenolic glycoside, Populus tremula × P. alba hybrid, Quinic acid, Salicaceae, Salicylate hydroxylase, Salicylic acid, nahG",

author = "Morse, \{Alison M.\} and Tschaplinski, \{Timothy J.\} and Christopher Dervinis and Pijut, \{Paula M.\} and Schmelz, \{Eric A.\} and Wendy Day and Davis, \{John M.\}",

year = "2007",

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doi = "10.1016/j.phytochem.2007.05.014",

language = "English",

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AU - Morse, Alison M.

AU - Tschaplinski, Timothy J.

AU - Dervinis, Christopher

AU - Pijut, Paula M.

AU - Schmelz, Eric A.

AU - Day, Wendy

AU - Davis, John M.

PY - 2007/8

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N2 - Metabolic profiling was used to investigate the molecular phenotypes of a transgenic Populus tremula × P. alba hybrid expressing the nahG transgene, a bacterial gene encoding salicylate hydroxylase that converts salicylic acid to catechol. Despite the efficacy of this transgenic approach to reduce salicylic acid levels in other model systems and thereby elucidate roles for salicylic acid in plant signaling, transgenic poplars had similar foliar levels of salicylic acid and catechol to that of non-transformed controls and exhibited no morphological phenotypes. To gain a deeper understanding of the basis for these observations, we analyzed metabolic profiles of leaves as influenced by transgene expression. Expression of nahG decreased quinic acid conjugates and increased catechol glucoside, while exerting little effect on levels of salicylic acid and catechol, the substrate and product, respectively, of the nahG enzyme. This suggests a biological role of elevated constitutive salicylic acid levels in Populus, in contrast to other plant systems in which nahG dramatically reduces salicylic acid levels.

AB - Metabolic profiling was used to investigate the molecular phenotypes of a transgenic Populus tremula × P. alba hybrid expressing the nahG transgene, a bacterial gene encoding salicylate hydroxylase that converts salicylic acid to catechol. Despite the efficacy of this transgenic approach to reduce salicylic acid levels in other model systems and thereby elucidate roles for salicylic acid in plant signaling, transgenic poplars had similar foliar levels of salicylic acid and catechol to that of non-transformed controls and exhibited no morphological phenotypes. To gain a deeper understanding of the basis for these observations, we analyzed metabolic profiles of leaves as influenced by transgene expression. Expression of nahG decreased quinic acid conjugates and increased catechol glucoside, while exerting little effect on levels of salicylic acid and catechol, the substrate and product, respectively, of the nahG enzyme. This suggests a biological role of elevated constitutive salicylic acid levels in Populus, in contrast to other plant systems in which nahG dramatically reduces salicylic acid levels.

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KW - Quinic acid

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KW - Salicylate hydroxylase

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Salicylate and catechol levels are maintained in nahG transgenic poplar

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