RVG peptide tethered bioreducible polyethylenimine for gene delivery to brain

Sejin Son; Do Won Hwang; Kaushik Singha; Ji Hoon Jeong; Tae Gwan Park; Dong Soo Lee; Won Jong Kim

doi:10.1016/j.jconrel.2010.08.011

RVG peptide tethered bioreducible polyethylenimine for gene delivery to brain

Sejin Son, Do Won Hwang, Kaushik Singha, Ji Hoon Jeong, Tae Gwan Park, Dong Soo Lee, Won Jong Kim

Research output: Contribution to journal › Article › peer-review

117 Scopus citations

Abstract

The work demonstrated the successful delivery of gene to mouse brain overcoming the blood-brain barrier (BBB) through expedient vector construct having RVG peptide as targeting ligand for neuronal cells. The newly developed delivery vector was designed to impart bioreducibility for greater intracellular pDNA release, higher serum stability and efficient complexing ability by incorporating disulfide linkage, PEG and low molecular weight polyethylenimine, respectively. The physiochemical properties of the polyplex, its cytotoxicity and the in vitro transfection efficiency on Neuro2a cell were studied prior to the successful in vivo study. In vivo fluorescence assay substantiated the permeation of the pDNA loaded polymeric vector through the BBB. The RVG-mediated target-specific cellular uptake of polymeric vector was established conclusively by competitive assay.

Original language	English
Pages (from-to)	18-25
Number of pages	8
Journal	Journal of Controlled Release
Volume	155
Issue number	1
DOIs	https://doi.org/10.1016/j.jconrel.2010.08.011
State	Published - Oct 10 2011
Externally published	Yes

Funding

This research was supported by Nano-Biotechnology Project (Regenomics, 20100002086 ), Bio R&D Program ( 20100019914 ), the Converging Research Center Program ( 2010K001366 ), the Brain Research Centre of the 21st Century Frontier Research Program ( 2009K001257 ), and the A3 (Korea -China -Japan) Program on Gene Delivery through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology , and a grant ( K00060-282 ) from the Fundamental R&D Program for Core Technology of Materials funded by the Ministry of Knowledge Economy, Republic of Korea .

Funders	Funder number
Brain Research Centre of the 21st Century Frontier Research Program	2009K001257
Converging Research Center Program	2010K001366
Japan) Program on Gene Delivery
Korea -China
Ministry of Knowledge Economy
National Research Foundation of Korea
Ministry of Education, Science and Technology	K00060-282

Keywords

Blood-brain barrier
Polyethylenimine
Polymeric gene delivery
RVG peptide

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jconrel.2010.08.011

Cite this

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abstract = "The work demonstrated the successful delivery of gene to mouse brain overcoming the blood-brain barrier (BBB) through expedient vector construct having RVG peptide as targeting ligand for neuronal cells. The newly developed delivery vector was designed to impart bioreducibility for greater intracellular pDNA release, higher serum stability and efficient complexing ability by incorporating disulfide linkage, PEG and low molecular weight polyethylenimine, respectively. The physiochemical properties of the polyplex, its cytotoxicity and the in vitro transfection efficiency on Neuro2a cell were studied prior to the successful in vivo study. In vivo fluorescence assay substantiated the permeation of the pDNA loaded polymeric vector through the BBB. The RVG-mediated target-specific cellular uptake of polymeric vector was established conclusively by competitive assay.",

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author = "Sejin Son and Hwang, {Do Won} and Kaushik Singha and Jeong, {Ji Hoon} and Park, {Tae Gwan} and Lee, {Dong Soo} and Kim, {Won Jong}",

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AU - Son, Sejin

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AU - Singha, Kaushik

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AU - Park, Tae Gwan

AU - Lee, Dong Soo

AU - Kim, Won Jong

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AB - The work demonstrated the successful delivery of gene to mouse brain overcoming the blood-brain barrier (BBB) through expedient vector construct having RVG peptide as targeting ligand for neuronal cells. The newly developed delivery vector was designed to impart bioreducibility for greater intracellular pDNA release, higher serum stability and efficient complexing ability by incorporating disulfide linkage, PEG and low molecular weight polyethylenimine, respectively. The physiochemical properties of the polyplex, its cytotoxicity and the in vitro transfection efficiency on Neuro2a cell were studied prior to the successful in vivo study. In vivo fluorescence assay substantiated the permeation of the pDNA loaded polymeric vector through the BBB. The RVG-mediated target-specific cellular uptake of polymeric vector was established conclusively by competitive assay.

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RVG peptide tethered bioreducible polyethylenimine for gene delivery to brain

Abstract

Funding

Keywords

UN SDGs

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