Rice actin binding protein RMD controls crown root angle in response to external phosphate

Guoqiang Huang; Wanqi Liang; Craig J. Sturrock; Bipin K. Pandey; Jitender Giri; Stefan Mairhofer; Daoyang Wang; Lukas Muller; Hexin Tan; Larry M. York; Jing Yang; Yu Song; Yu Jin Kim; Yang Qiao; Jian Xu; Stefan Kepinski; Malcolm J. Bennett; Dabing Zhang

doi:10.1038/s41467-018-04710-x

Rice actin binding protein RMD controls crown root angle in response to external phosphate

Guoqiang Huang, Wanqi Liang, Craig J. Sturrock, Bipin K. Pandey, Jitender Giri, Stefan Mairhofer, Daoyang Wang, Lukas Muller, Hexin Tan, Larry M. York, Jing Yang, Yu Song, Yu Jin Kim, Yang Qiao, Jian Xu, Stefan Kepinski, Malcolm J. Bennett, Dabing Zhang

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79 Scopus citations

Abstract

Root angle has a major impact on acquisition of nutrients like phosphate that accumulate in topsoil and in many species; low phosphate induces shallower root growth as an adaptive response. Identifying genes and mechanisms controlling root angle is therefore of paramount importance to plant breeding. Here we show that the actin-binding protein Rice Morphology Determinant (RMD) controls root growth angle by linking actin filaments and gravity-sensing organelles termed statoliths. RMD is upregulated in response to low external phosphate and mutants lacking of RMD have steeper crown root growth angles that are unresponsive to phosphate levels. RMD protein localizes to the surface of statoliths, and rmd mutants exhibit faster gravitropic response owing to more rapid statoliths movement. We conclude that adaptive changes to root angle in response to external phosphate availability are RMD dependent, providing a potential target for breeders.

Original language	English
Article number	2346
Journal	Nature Communications
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41467-018-04710-x
State	Published - Dec 1 2018
Externally published	Yes

Funding

We thank Miss Mingjiao Chen and Mr Zhijing Luo for performing rice crosses and rice cultivation. We thank the Instrumental Analysis Center of Shanghai Jiao Tong University (SJTU) for their help in obtaining and analyzing TEM images. This work was supported by the National Key Research and Development Program of China (2016YFD0100804); National Natural Science Foundation of China (grant no. 31430009, 31230051, 31322040, and 31271698); the Innovative Research Team, Ministry of Education, and 111 Project (grant no. B14016); the Science and Technology Commission of Shanghai Municipality (grant no. 13JC1408200); the National Transgenic Major Program (grant no. 2016ZX08009003-003-007); The European Research Council (ERC) FUTURE-ROOTS Advanced Investigator grant; Royal Society Wolfson, Newton Bhabha and Advanced Fellowship awards; University of Nottingham Beacon of Excellence PhD +fellowship; The Interuniversity Attraction Poles Programme (IUAP P7/29 \u201CMARS\u201D), initiated by the Belgian Science Policy Office; National Key Laboratory of Crop Genetic Improvement; The DBT-CREST fellowship (to J.G.) and the National Research Foundation Singapore under its Competitive Research Programme (CRP Award No. NRF2010NRF-CRP002-018).

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Huang, G., Liang, W., Sturrock, C. J., Pandey, B. K., Giri, J., Mairhofer, S., Wang, D., Muller, L., Tan, H., York, L. M., Yang, J., Song, Y., Kim, Y. J., Qiao, Y., Xu, J., Kepinski, S., Bennett, M. J., & Zhang, D. (2018). Rice actin binding protein RMD controls crown root angle in response to external phosphate. Nature Communications, 9(1), Article 2346. https://doi.org/10.1038/s41467-018-04710-x

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title = "Rice actin binding protein RMD controls crown root angle in response to external phosphate",

abstract = "Root angle has a major impact on acquisition of nutrients like phosphate that accumulate in topsoil and in many species; low phosphate induces shallower root growth as an adaptive response. Identifying genes and mechanisms controlling root angle is therefore of paramount importance to plant breeding. Here we show that the actin-binding protein Rice Morphology Determinant (RMD) controls root growth angle by linking actin filaments and gravity-sensing organelles termed statoliths. RMD is upregulated in response to low external phosphate and mutants lacking of RMD have steeper crown root growth angles that are unresponsive to phosphate levels. RMD protein localizes to the surface of statoliths, and rmd mutants exhibit faster gravitropic response owing to more rapid statoliths movement. We conclude that adaptive changes to root angle in response to external phosphate availability are RMD dependent, providing a potential target for breeders.",

author = "Guoqiang Huang and Wanqi Liang and Sturrock, {Craig J.} and Pandey, {Bipin K.} and Jitender Giri and Stefan Mairhofer and Daoyang Wang and Lukas Muller and Hexin Tan and York, {Larry M.} and Jing Yang and Yu Song and Kim, {Yu Jin} and Yang Qiao and Jian Xu and Stefan Kepinski and Bennett, {Malcolm J.} and Dabing Zhang",

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Huang, G, Liang, W, Sturrock, CJ, Pandey, BK, Giri, J, Mairhofer, S, Wang, D, Muller, L, Tan, H, York, LM, Yang, J, Song, Y, Kim, YJ, Qiao, Y, Xu, J, Kepinski, S, Bennett, MJ & Zhang, D 2018, 'Rice actin binding protein RMD controls crown root angle in response to external phosphate', Nature Communications, vol. 9, no. 1, 2346. https://doi.org/10.1038/s41467-018-04710-x

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AU - Huang, Guoqiang

AU - Liang, Wanqi

AU - Sturrock, Craig J.

AU - Pandey, Bipin K.

AU - Giri, Jitender

AU - Mairhofer, Stefan

AU - Wang, Daoyang

AU - Muller, Lukas

AU - Tan, Hexin

AU - York, Larry M.

AU - Yang, Jing

AU - Song, Yu

AU - Kim, Yu Jin

AU - Qiao, Yang

AU - Xu, Jian

AU - Kepinski, Stefan

AU - Bennett, Malcolm J.

AU - Zhang, Dabing

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Root angle has a major impact on acquisition of nutrients like phosphate that accumulate in topsoil and in many species; low phosphate induces shallower root growth as an adaptive response. Identifying genes and mechanisms controlling root angle is therefore of paramount importance to plant breeding. Here we show that the actin-binding protein Rice Morphology Determinant (RMD) controls root growth angle by linking actin filaments and gravity-sensing organelles termed statoliths. RMD is upregulated in response to low external phosphate and mutants lacking of RMD have steeper crown root growth angles that are unresponsive to phosphate levels. RMD protein localizes to the surface of statoliths, and rmd mutants exhibit faster gravitropic response owing to more rapid statoliths movement. We conclude that adaptive changes to root angle in response to external phosphate availability are RMD dependent, providing a potential target for breeders.

AB - Root angle has a major impact on acquisition of nutrients like phosphate that accumulate in topsoil and in many species; low phosphate induces shallower root growth as an adaptive response. Identifying genes and mechanisms controlling root angle is therefore of paramount importance to plant breeding. Here we show that the actin-binding protein Rice Morphology Determinant (RMD) controls root growth angle by linking actin filaments and gravity-sensing organelles termed statoliths. RMD is upregulated in response to low external phosphate and mutants lacking of RMD have steeper crown root growth angles that are unresponsive to phosphate levels. RMD protein localizes to the surface of statoliths, and rmd mutants exhibit faster gravitropic response owing to more rapid statoliths movement. We conclude that adaptive changes to root angle in response to external phosphate availability are RMD dependent, providing a potential target for breeders.

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Rice actin binding protein RMD controls crown root angle in response to external phosphate

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