Responses of bone-forming cells on pre-immersed Zr-based bulk metallic glasses: Effects of composition and roughness

L. Huang; Z. Cao; H. M. Meyer; P. K. Liaw; E. Garlea; J. R. Dunlap; T. Zhang; W. He

doi:10.1016/j.actbio.2010.08.002

Responses of bone-forming cells on pre-immersed Zr-based bulk metallic glasses: Effects of composition and roughness

L. Huang, Z. Cao, H. M. Meyer, P. K. Liaw, E. Garlea, J. R. Dunlap, T. Zhang, W. He

Surface Chemistry & Catalysis Group

Research output: Contribution to journal › Article › peer-review

103 Scopus citations

Abstract

Bulk metallic glasses (BMGs) demonstrate attractive properties for potential biomedical applications, owing to their amorphous structure. The present work has investigated the biocompatibility of Zr-based BMGs by studying the cellular behavior of bone-forming mouse MC3T3-E1 pre-osteoblast cells. A Ti-6Al-4V alloy was used as a reference material. Pre-immersion treatment was performed on BMG samples in phosphate-buffered saline prior to cell experiments. The effects of 1 at.% yttrium alloying and surface roughness on cellular behavior were examined. The general biosafety of Zr-based BMGs for MC3T3-E1 cells was revealed as normal cell responses. Pre-immersion treatment was found to effectively reduce the surface concentrations of alloying elements. Micro-alloying with 1 at.% yttrium did not significantly affect cell adhesion and proliferation, but slightly decreased alkaline phosphatase (ALP) activity on rough surfaces. Lower cell adhesion and proliferation were found on smooth surfaces of Zr-based BMGs compared to their rougher counterparts. Higher ALP activity was detected on rougher surfaces. To obtain a mechanistic understanding surface free energy was correlated with cell adhesion.

Original language	English
Pages (from-to)	395-405
Number of pages	11
Journal	Acta Biomaterialia
Volume	7
Issue number	1
DOIs	https://doi.org/10.1016/j.actbio.2010.08.002
State	Published - Jan 2011

Funding

The authors are grateful to Drs. Scott C. Lenaghan and David A. Gerard for their kind suggestions on SEM fixation procedures and Mr. Sameer Paital for his assistance in contact angle tests. This work was financially supported by the National Science Foundation International Materials Institutes Program ( DMR-0231320 ) and National Nature Science Foundation of China (Grants Nos. 50771005 and 50631010 ). Research at the Oak Ridge National Laboratory SHaRE User Facility was sponsored by the Scientific User Facilities Division, Office of Basic Energy Sciences, US Department of Energy.

Funders	Funder number
National Science Foundation International Materials Institutes Program	DMR-0231320
Scientific User Facilities Division
U.S. Department of Energy
Basic Energy Sciences
National Natural Science Foundation of China	50631010, 50771005

Keywords

Biocompatibility
Bulk metallic glass
Osteoblast
Pre-immersion
Surface roughness

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10.1016/j.actbio.2010.08.002

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title = "Responses of bone-forming cells on pre-immersed Zr-based bulk metallic glasses: Effects of composition and roughness",

abstract = "Bulk metallic glasses (BMGs) demonstrate attractive properties for potential biomedical applications, owing to their amorphous structure. The present work has investigated the biocompatibility of Zr-based BMGs by studying the cellular behavior of bone-forming mouse MC3T3-E1 pre-osteoblast cells. A Ti-6Al-4V alloy was used as a reference material. Pre-immersion treatment was performed on BMG samples in phosphate-buffered saline prior to cell experiments. The effects of 1 at.% yttrium alloying and surface roughness on cellular behavior were examined. The general biosafety of Zr-based BMGs for MC3T3-E1 cells was revealed as normal cell responses. Pre-immersion treatment was found to effectively reduce the surface concentrations of alloying elements. Micro-alloying with 1 at.% yttrium did not significantly affect cell adhesion and proliferation, but slightly decreased alkaline phosphatase (ALP) activity on rough surfaces. Lower cell adhesion and proliferation were found on smooth surfaces of Zr-based BMGs compared to their rougher counterparts. Higher ALP activity was detected on rougher surfaces. To obtain a mechanistic understanding surface free energy was correlated with cell adhesion.",

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AU - Dunlap, J. R.

AU - Zhang, T.

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AB - Bulk metallic glasses (BMGs) demonstrate attractive properties for potential biomedical applications, owing to their amorphous structure. The present work has investigated the biocompatibility of Zr-based BMGs by studying the cellular behavior of bone-forming mouse MC3T3-E1 pre-osteoblast cells. A Ti-6Al-4V alloy was used as a reference material. Pre-immersion treatment was performed on BMG samples in phosphate-buffered saline prior to cell experiments. The effects of 1 at.% yttrium alloying and surface roughness on cellular behavior were examined. The general biosafety of Zr-based BMGs for MC3T3-E1 cells was revealed as normal cell responses. Pre-immersion treatment was found to effectively reduce the surface concentrations of alloying elements. Micro-alloying with 1 at.% yttrium did not significantly affect cell adhesion and proliferation, but slightly decreased alkaline phosphatase (ALP) activity on rough surfaces. Lower cell adhesion and proliferation were found on smooth surfaces of Zr-based BMGs compared to their rougher counterparts. Higher ALP activity was detected on rougher surfaces. To obtain a mechanistic understanding surface free energy was correlated with cell adhesion.

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Responses of bone-forming cells on pre-immersed Zr-based bulk metallic glasses: Effects of composition and roughness

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