Reproducible automated renewable column generation

Shalina C. Metzger; Brian W. Ticknor; Kayron N. Tevepaugh; Debra A. Bostick; Eddy H. McBay; M. Paul Field; Hwan Kim; Cole R. Hexel

doi:10.1080/01496395.2019.1577439

Reproducible automated renewable column generation

Shalina C. Metzger, Brian W. Ticknor, Kayron N. Tevepaugh, Debra A. Bostick, Eddy H. McBay, M. Paul Field, Hwan Kim, Cole R. Hexel

Chemical & Isotopic Mass Spectrometry

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Preparation of chromatographic columns requires considerable time during chemical separation procedures. Automated systems capable of repeatedly generating renewable, uniformly packed columns would find widespread use in analytical laboratories. Here, a fully automated commercial-off-the-shelf (COTS) system was utilized to pack and unpack 1 mL TEVA and UTEVA columns. To accurately determine the reproducibility of the resin load per column, studies were conducted over several weeks. Method optimization yielded an improvement in loading from 15–19% to 3–5% relative standard deviation. Availability of automated COTS equipment capable of reproducibly generating separation columns has the potential to reduce time and costs associated with column separation.

Original language	English
Pages (from-to)	860-866
Number of pages	7
Journal	Separation Science and Technology (Philadelphia)
Volume	55
Issue number	5
DOIs	https://doi.org/10.1080/01496395.2019.1577439
State	Published - Mar 23 2020

Funding

This work is supported by the Safeguards Technology Development Program, Office of Nonproliferation and Arms Control, at the Department of Energy’s National Nuclear Security Administration under contract [DE-AC05-00OR22725] with UT-Battelle, LLC. Oak Ridge National Laboratory is managed by UT-Battelle for the Department of Energy under Contract [DE-AC05-000R22725];Office of Defense Nuclear Nonproliferation [DE-AC05-00OR22725].

Funders	Funder number
Office of Nonproliferation and Arms Control
Safeguards Technology Development Program
U.S. Department of Energy	DE-AC05-000R22725
National Nuclear Security Administration	DE-AC05-00OR22725
Office of Defense Nuclear Nonproliferation
Oak Ridge National Laboratory
UT-Battelle

Keywords

Renewable column
automated
column packing
safeguards

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10.1080/01496395.2019.1577439

Cite this

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title = "Reproducible automated renewable column generation",

abstract = "Preparation of chromatographic columns requires considerable time during chemical separation procedures. Automated systems capable of repeatedly generating renewable, uniformly packed columns would find widespread use in analytical laboratories. Here, a fully automated commercial-off-the-shelf (COTS) system was utilized to pack and unpack 1 mL TEVA and UTEVA columns. To accurately determine the reproducibility of the resin load per column, studies were conducted over several weeks. Method optimization yielded an improvement in loading from 15–19% to 3–5% relative standard deviation. Availability of automated COTS equipment capable of reproducibly generating separation columns has the potential to reduce time and costs associated with column separation.",

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AU - Metzger, Shalina C.

AU - Ticknor, Brian W.

AU - Tevepaugh, Kayron N.

AU - Bostick, Debra A.

AU - McBay, Eddy H.

AU - Field, M. Paul

AU - Kim, Hwan

AU - Hexel, Cole R.

PY - 2020/3/23

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AB - Preparation of chromatographic columns requires considerable time during chemical separation procedures. Automated systems capable of repeatedly generating renewable, uniformly packed columns would find widespread use in analytical laboratories. Here, a fully automated commercial-off-the-shelf (COTS) system was utilized to pack and unpack 1 mL TEVA and UTEVA columns. To accurately determine the reproducibility of the resin load per column, studies were conducted over several weeks. Method optimization yielded an improvement in loading from 15–19% to 3–5% relative standard deviation. Availability of automated COTS equipment capable of reproducibly generating separation columns has the potential to reduce time and costs associated with column separation.

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Reproducible automated renewable column generation

Abstract

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Keywords

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