Abstract
The evaluation of reproducibility in analysis of complex peptide mixtures generated from whole cell lysates was discussed. LC-MS on an electrospray ion trap mass spectrometer (ITMS) and an electrospray Fourier-transform mass spectrometer (FT-MS) were used for the generation of complex peptide mixtures from whole cell lysates. The analysis of whole cell lysates by LC-MS/MS on an ITMS and LC-MS on an FT-MS were used for more accurate analysis of proteomes. The experiment consisted of five replicate injections by an autosampler on to a C18 nanocolumn system connected to a Finnigan nanospray source on the ITMS.
| Original language | English |
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| Pages | 261-262 |
| Number of pages | 2 |
| State | Published - 2002 |
| Event | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002 |
Conference
| Conference | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics |
|---|---|
| Country/Territory | United States |
| City | Orlando, FL |
| Period | 06/2/02 → 06/6/02 |