Related methods for three-dimensional imaging

J. Michael Tyszka; Seth W. Ruffins; Jamey P. Weichert; Michael J. Paulus; Scott E. Fraser

doi:10.1007/978-0-387-45524-2_34

Related methods for three-dimensional imaging

J. Michael Tyszka, Seth W. Ruffins, Jamey P. Weichert, Michael J. Paulus, Scott E. Fraser

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

The ability of confocal laser-scanning microscopy to collect stacks of optical sections has made three-dimensional (volumetric) imaging a standard analytical tool in experimental cell and developmental biology. Parallel developments in deconvolution techniques, especially as computational power increased and costs decreased, offered tools to make three-dimensional (3D) imaging from widefield as well as confocal microscopes possible. Despite the high spatial resolution provided by these 3D methods, they all suffer from a common limitation: light scattering in the specimen limits them to operating in the outer few hundred micrometers of the specimen.

Original language	English
Title of host publication	Handbook of Biological Confocal Microscopy
Subtitle of host publication	Third Edition
Publisher	Springer US
Pages	607-626
Number of pages	20
ISBN (Print)	038725921X, 9780387259215
DOIs	https://doi.org/10.1007/978-0-387-45524-2_34
State	Published - 2006
Externally published	Yes

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10.1007/978-0-387-45524-2_34

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AU - Ruffins, Seth W.

AU - Weichert, Jamey P.

AU - Paulus, Michael J.

AU - Fraser, Scott E.

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AB - The ability of confocal laser-scanning microscopy to collect stacks of optical sections has made three-dimensional (volumetric) imaging a standard analytical tool in experimental cell and developmental biology. Parallel developments in deconvolution techniques, especially as computational power increased and costs decreased, offered tools to make three-dimensional (3D) imaging from widefield as well as confocal microscopes possible. Despite the high spatial resolution provided by these 3D methods, they all suffer from a common limitation: light scattering in the specimen limits them to operating in the outer few hundred micrometers of the specimen.

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ER -

Related methods for three-dimensional imaging

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