Abstract
Different phenomena occurring during capillary electrophoretic (CE) separations (i.e., heat generation, spontaneous injection, siphoning effect and adsorption of the solute on the capillary wall) were investigated in cylindrical and rectangular capillaries of large cross-sectional area. The equation that relates the plate height to the extent of adsorption in a rectangular duct is shown and compared with that for cylindrical capillaries. Theoretical and experimental results obtained with capillaries of both geometries are compared. The advantages and drawbacks of using rectangular capillaries in micropreparative CE are discussed. A simple modification to the commercial CE instrument used in this work allowed the easy installation of rectangular capillaries within the apparatus.
| Original language | English |
|---|---|
| Pages (from-to) | 243-253 |
| Number of pages | 11 |
| Journal | Journal of Chromatography A |
| Volume | 737 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jun 21 1996 |
| Externally published | Yes |
Funding
The authors thank Professor H. Poppe, Dr. J.C. Diez-Masa and Dr. M. de Frutos for stimulating discussions and J.M. Campos for the modification of the cartridge cover. Financial support of this work by CICYT (Spain), grant AMB92-0515, and the Local Government of the Canary Islands is acknowledged.
| Funders | Funder number |
|---|---|
| Comisión Interministerial de Ciencia y Tecnología | AMB92-0515 |
Keywords
- adsorption
- dispersive effects
- heat generation
- rectangular capillaries
- siphoning effect