Recombinant and in vitro expression systems for hydrogenases: New frontiers in basic and applied studies for biological and synthetic H2 production

Christine M. English; Carrie Eckert; Katherine Brown; Michael Seibert; Paul W. King

doi:10.1039/b913426n

Recombinant and in vitro expression systems for hydrogenases: New frontiers in basic and applied studies for biological and synthetic H₂ production

Christine M. English, Carrie Eckert, Katherine Brown, Michael Seibert, Paul W. King

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

This review focuses on recent progress in developing heterologous and recombinant expression as well as in vitro maturation systems for the biosynthesis of active [FeFe] and [NiFe]-hydrogenases, which catalyze the reversible reaction, H₂↔ 2e- + 2H⁺. Activities of [FeFe] and [NiFe]-hydrogenases produced from different recombinant and in vitro maturation approaches are compared. Examples of how hydrogenase expression supports basic and applied studies of these enzymes are presented, and barriers to achieving more viable biological and synthetic H₂-production systems and catalysts are addressed.

Original language	English
Pages (from-to)	9970-9978
Number of pages	9
Journal	Journal of the Chemical Society - Dalton Transactions
Issue number	45
DOIs	https://doi.org/10.1039/b913426n
State	Published - Nov 10 2009
Externally published	Yes

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abstract = "This review focuses on recent progress in developing heterologous and recombinant expression as well as in vitro maturation systems for the biosynthesis of active [FeFe] and [NiFe]-hydrogenases, which catalyze the reversible reaction, H2↔ 2e- + 2H+. Activities of [FeFe] and [NiFe]-hydrogenases produced from different recombinant and in vitro maturation approaches are compared. Examples of how hydrogenase expression supports basic and applied studies of these enzymes are presented, and barriers to achieving more viable biological and synthetic H2-production systems and catalysts are addressed.",

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Recombinant and in vitro expression systems for hydrogenases: New frontiers in basic and applied studies for biological and synthetic H₂ production. / English, Christine M.; Eckert, Carrie; Brown, Katherine et al.
In: Journal of the Chemical Society - Dalton Transactions, No. 45, 10.11.2009, p. 9970-9978.

Research output: Contribution to journal › Article › peer-review

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Recombinant and in vitro expression systems for hydrogenases: New frontiers in basic and applied studies for biological and synthetic H₂ production

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