Recent developments in primer design for DNA polymorphism and mRNA profiling in higher plants

Xiaohan Yang; Brian E. Scheffler; Leslie A. Weston

doi:10.1186/1746-4811-2-4

Recent developments in primer design for DNA polymorphism and mRNA profiling in higher plants

Xiaohan Yang, Brian E. Scheffler, Leslie A. Weston

Research output: Contribution to journal › Review article › peer-review

10 Scopus citations

Abstract

Primer design is a critical step in the application of PCR-based technologies in gene expression and genetic diversity analysis. As more plant genomes have been sequenced in recent years, the emphasis of primer design strategy has shifted to genome-wide and high-throughput direction. This paper summarizes recent advances in primer design for profiling of DNA polymorphism and mRNA in higher plants, as well as new primer systems developed for animals that can be adapted for plants.

Original language	English
Article number	4
Journal	Plant Methods
Volume	2
Issue number	1
DOIs	https://doi.org/10.1186/1746-4811-2-4
State	Published - Mar 1 2006
Externally published	Yes

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10.1186/1746-4811-2-4

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title = "Recent developments in primer design for DNA polymorphism and mRNA profiling in higher plants",

abstract = "Primer design is a critical step in the application of PCR-based technologies in gene expression and genetic diversity analysis. As more plant genomes have been sequenced in recent years, the emphasis of primer design strategy has shifted to genome-wide and high-throughput direction. This paper summarizes recent advances in primer design for profiling of DNA polymorphism and mRNA in higher plants, as well as new primer systems developed for animals that can be adapted for plants.",

author = "Xiaohan Yang and Scheffler, {Brian E.} and Weston, {Leslie A.}",

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AU - Yang, Xiaohan

AU - Scheffler, Brian E.

AU - Weston, Leslie A.

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N2 - Primer design is a critical step in the application of PCR-based technologies in gene expression and genetic diversity analysis. As more plant genomes have been sequenced in recent years, the emphasis of primer design strategy has shifted to genome-wide and high-throughput direction. This paper summarizes recent advances in primer design for profiling of DNA polymorphism and mRNA in higher plants, as well as new primer systems developed for animals that can be adapted for plants.

AB - Primer design is a critical step in the application of PCR-based technologies in gene expression and genetic diversity analysis. As more plant genomes have been sequenced in recent years, the emphasis of primer design strategy has shifted to genome-wide and high-throughput direction. This paper summarizes recent advances in primer design for profiling of DNA polymorphism and mRNA in higher plants, as well as new primer systems developed for animals that can be adapted for plants.

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U2 - 10.1186/1746-4811-2-4

DO - 10.1186/1746-4811-2-4

M3 - Review article

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SN - 1746-4811

VL - 2

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Recent developments in primer design for DNA polymorphism and mRNA profiling in higher plants

Abstract

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