Really smart bioconjugates of smart polymers and receptor proteins

Allan S. Hoffman, Patrick S. Stayton, Volga Bulmus, Guohua Chen, Jingping Chen, Chuck Cheung, Ashutosh Chilkoti, Zhongli Ding, Liangchang Dong, Robin Fong, Chantal A. Lackey, Cynthia J. Long, Morikazu Miura, John E. Morris, Niren Murthy, Yoshikuni Nabeshima, Tae Gwan Park, Ollie W. Press, Tsuyoshi Shimoboji, Sara ShoemakerHeung Joon Yang, Nobuo Monji, Robert C. Nowinski, Carole Ann Cole, John H. Priest, J. Milton Harris, Katsuhiko Nakamae, Takashi Nishino, Takashi Miyata

Research output: Contribution to journalArticlepeer-review

309 Scopus citations

Abstract

Over the past 18 years we have been deeply involved with the synthesis and applications of stimuli-responsive polymer systems, especially polymer-biomolecule conjugates. This article summarizes our work with one of these conjugate systems, specifically polymer-protein conjugates. We include conjugates prepared by random polymer conjugation to lysine amino groups, and also those prepared by site-specific conjugation of the polymer to specific amino acid sites that are genetically engineered into the known amino acid sequence of the protein. We describe the preparation and properties of thermally sensitive random conjugates to enzymes and several affinity recognition proteins. We have also prepared site-specific conjugates to streptavidin with temperature-sensitive polymers, pH-sensitive polymers, and light-sensitive polymers. The preparation of these conjugates and their many fascinating applications are reviewed in this article. (C) 2000 John Wiley and Sons, Inc.

Original languageEnglish
Pages (from-to)577-586
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume52
Issue number4
DOIs
StatePublished - Dec 2000
Externally publishedYes

Keywords

  • Affinity separation
  • Bioconjugates
  • Immunoassay
  • Intelligent polymers
  • Polymer conjugation
  • Recognition proteins
  • Site-specific conjugation
  • Smart polymers
  • Stimuli-responsive polymers

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