Rapid analysis of isomeric exogenous metabolites by differential mobility spectrometry - Mass spectrometry

Whitney B. Parson, Bradley B. Schneider, Vilmos Kertesz, Jay J. Corr, Thomas R. Covey, Gary J. Van Berkel

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

The direct separation of isomeric glucuronide metabolites from propranolol dosed tissue extracts by differential mobility spectrometry - mass spectrometry (DMS-MS) with the use of the polar gas-phase chemical modifier acetonitrile was demonstrated. The DMS gas-phase separation was able to resolve the isomeric metabolites with separation times on the order of milliseconds instead of minutes which is typically required when using pre-ionization chromatographic separation methods. Direct separation of isomeric metabolites from the complex tissue extract was confirmed by implementing a high-performance liquid chromatography (HPLC) separation prior to the DMS-MS analysis to pre-separate the species of interest. The ability to separate isomeric exogenous metabolites directly from a complex tissue extract is expected to facilitate the drug development process by increasing analytical throughput without the requirement for pre-ionization cleanup or separation strategies. Published in 2011 by John Wiley & Sons, Ltd.

Original languageEnglish
Pages (from-to)3382-3386
Number of pages5
JournalRapid Communications in Mass Spectrometry
Volume25
Issue number22
DOIs
StatePublished - Nov 30 2011

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