RACK1 mediates multiple hormone responsiveness and developmental processes in Arabidopsis

Jin Gui Chen; Hemayet Ullah; Brenda Temple; Jiansheng Liang; Jianjun Guo; José M. Alonso; Joseph R. Ecker; Alan M. Jones

doi:10.1093/jxb/erl035

RACK1 mediates multiple hormone responsiveness and developmental processes in Arabidopsis

Jin Gui Chen, Hemayet Ullah, Brenda Temple, Jiansheng Liang, Jianjun Guo, José M. Alonso, Joseph R. Ecker, Alan M. Jones

Research output: Contribution to journal › Article › peer-review

123 Scopus citations

Abstract

The scaffold protein RACK1 (Receptor for Activated C Kinase 1) serves as an integrative point for diverse signal transduction pathways. The Arabidopsis genome contains three RACK1 orthologues, however, little is known about their functions. It is reported here that one member of this gene family, RACK1A, previously identified as the Arabidopsis homologue of the tobacco arcA gene, mediates hormone responses and plays a regulatory role in multiple developmental processes. RACK1A expresses ubiquitously in Arabidopsis. Loss-of-function mutations in RACK1A confer defects in multiple developmental processes including seed germination, leaf production, and flowering. rack1a mutants displayed reduced sensitivity to gibberellin and brassinosteroid in seed germination, hypersensitivity to abscisic acid in seed germination and early seedling development, and hyposensitivity to auxin in adventitious and lateral root formation. These results provide the first genetic evidence that RACK1A is involved in multiple signal transduction pathways.

Original language	English
Pages (from-to)	2697-2708
Number of pages	12
Journal	Journal of Experimental Botany
Volume	57
Issue number	11
DOIs	https://doi.org/10.1093/jxb/erl035
State	Published - Aug 2006
Externally published	Yes

Funding

This work was supported by grants from the Natural Sciences and Engineering Research Council of Canada, the Canada Foundation for Innovation, the British Columbia Ministry of Advanced Education, the University of British Columbia, and the National Natural Science Foundation of China (grant no. 30528023) to J-GC; by grants from the NIGMS (GM65989-01) and the NSF (MCB-0209711) to AMJ, and in part by a grant from the National Key Basic Research and Development Program of China (2003CB114300303) to JL. Isolation of the rack1a-1 and rack1a-2 alleles was made possible by an NSF grant (0115103) to JRE.

Funders	Funder number
British Columbia Ministry of Advanced Education
National Key Basic Research and Development program of China	0115103, 2003CB114300303
National Science Foundation	MCB-0209711
National Cancer Institute	P30CA016086
National Institute of General Medical Sciences	GM65989-01
Natural Sciences and Engineering Research Council of Canada
Canada Foundation for Innovation
National Natural Science Foundation of China	30528023
University of British Columbia

Keywords

Abscisic acid
Auxin
Brassinosteroid
Flowering
Gibberellin
RACK1
Seed germination
Signal transduction

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10.1093/jxb/erl035

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title = "RACK1 mediates multiple hormone responsiveness and developmental processes in Arabidopsis",

abstract = "The scaffold protein RACK1 (Receptor for Activated C Kinase 1) serves as an integrative point for diverse signal transduction pathways. The Arabidopsis genome contains three RACK1 orthologues, however, little is known about their functions. It is reported here that one member of this gene family, RACK1A, previously identified as the Arabidopsis homologue of the tobacco arcA gene, mediates hormone responses and plays a regulatory role in multiple developmental processes. RACK1A expresses ubiquitously in Arabidopsis. Loss-of-function mutations in RACK1A confer defects in multiple developmental processes including seed germination, leaf production, and flowering. rack1a mutants displayed reduced sensitivity to gibberellin and brassinosteroid in seed germination, hypersensitivity to abscisic acid in seed germination and early seedling development, and hyposensitivity to auxin in adventitious and lateral root formation. These results provide the first genetic evidence that RACK1A is involved in multiple signal transduction pathways.",

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AU - Temple, Brenda

AU - Liang, Jiansheng

AU - Guo, Jianjun

AU - Alonso, José M.

AU - Ecker, Joseph R.

AU - Jones, Alan M.

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KW - Abscisic acid

KW - Auxin

KW - Brassinosteroid

KW - Flowering

KW - Gibberellin

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KW - Signal transduction

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RACK1 mediates multiple hormone responsiveness and developmental processes in Arabidopsis

Abstract

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