Quantitation of repaglinide and metabolites in mouse whole-body thin tissue sections using droplet-based liquid microjunction surface sampling-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry

Weiqi Chen; Lifei Wang; Gary J. Van Berkel; Vilmos Kertesz; Jinping Gan

doi:10.1016/j.chroma.2015.10.093

Quantitation of repaglinide and metabolites in mouse whole-body thin tissue sections using droplet-based liquid microjunction surface sampling-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry

Weiqi Chen, Lifei Wang, Gary J. Van Berkel, Vilmos Kertesz, Jinping Gan

Bioanalytical Mass Spectrometry

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Herein, quantitation aspects of a fully automated autosampler/HPLC-MS/MS system applied for unattended droplet-based surface sampling of repaglinide dosed thin tissue sections with subsequent HPLC separation and mass spectrometric analysis of parent drug and various drug metabolites were studied. Major organs (brain, lung, liver, kidney and muscle) from whole-body thin tissue sections and corresponding organ homogenates prepared from repaglinide dosed mice were sampled by surface sampling and by bulk extraction, respectively, and analyzed by HPLC-MS/MS. A semi-quantitative agreement between data obtained by surface sampling and that by employing organ homogenate extraction was observed. Drug concentrations obtained by the two methods followed the same patterns for post-dose time points (0.25, 0.5, 1 and 2 h). Drug amounts determined in the specific tissues was typically higher when analyzing extracts from the organ homogenates. In addition, relative comparison of the levels of individual metabolites between the two analytical methods also revealed good semi-quantitative agreement.

Original language	English
Pages (from-to)	137-143
Number of pages	7
Journal	Journal of Chromatography A
Volume	1439
DOIs	https://doi.org/10.1016/j.chroma.2015.10.093
State	Published - Mar 25 2016

Funding

W.C., L.W. and J.G. would like to thank Mr. Markus Fancher and the Technical Support Unit at Bristol-Myers Squibb for assistance in animal handling and dosing. Support for V.K. and G.J.V.B. was provided through a Cooperative Research and Development Agreement with Sciex (CRADA NFE-10-02966). The software package LMJ Points Plus © V2.98 was previously developed with support from the U.S. Department of Energy, Office of Science, Basic Energy Sciences, Chemical Sciences, Geosciences, and Biosciences Division.

Keywords

Autosampler
Droplet-based liquid extraction
Liquid microjunction
Quantitation
Repaglinide
Spatial distribution

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Chen, W., Wang, L., Van Berkel, G. J., Kertesz, V., & Gan, J. (2016). Quantitation of repaglinide and metabolites in mouse whole-body thin tissue sections using droplet-based liquid microjunction surface sampling-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry. Journal of Chromatography A, 1439, 137-143. https://doi.org/10.1016/j.chroma.2015.10.093

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title = "Quantitation of repaglinide and metabolites in mouse whole-body thin tissue sections using droplet-based liquid microjunction surface sampling-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry",

abstract = "Herein, quantitation aspects of a fully automated autosampler/HPLC-MS/MS system applied for unattended droplet-based surface sampling of repaglinide dosed thin tissue sections with subsequent HPLC separation and mass spectrometric analysis of parent drug and various drug metabolites were studied. Major organs (brain, lung, liver, kidney and muscle) from whole-body thin tissue sections and corresponding organ homogenates prepared from repaglinide dosed mice were sampled by surface sampling and by bulk extraction, respectively, and analyzed by HPLC-MS/MS. A semi-quantitative agreement between data obtained by surface sampling and that by employing organ homogenate extraction was observed. Drug concentrations obtained by the two methods followed the same patterns for post-dose time points (0.25, 0.5, 1 and 2 h). Drug amounts determined in the specific tissues was typically higher when analyzing extracts from the organ homogenates. In addition, relative comparison of the levels of individual metabolites between the two analytical methods also revealed good semi-quantitative agreement.",

keywords = "Autosampler, Droplet-based liquid extraction, Liquid microjunction, Quantitation, Repaglinide, Spatial distribution",

author = "Weiqi Chen and Lifei Wang and {Van Berkel}, {Gary J.} and Vilmos Kertesz and Jinping Gan",

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year = "2016",

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doi = "10.1016/j.chroma.2015.10.093",

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Chen, W, Wang, L, Van Berkel, GJ, Kertesz, V & Gan, J 2016, 'Quantitation of repaglinide and metabolites in mouse whole-body thin tissue sections using droplet-based liquid microjunction surface sampling-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry', Journal of Chromatography A, vol. 1439, pp. 137-143. https://doi.org/10.1016/j.chroma.2015.10.093

Quantitation of repaglinide and metabolites in mouse whole-body thin tissue sections using droplet-based liquid microjunction surface sampling-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry. / Chen, Weiqi; Wang, Lifei; Van Berkel, Gary J. et al.
In: Journal of Chromatography A, Vol. 1439, 25.03.2016, p. 137-143.

Research output: Contribution to journal › Article › peer-review

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AU - Chen, Weiqi

AU - Wang, Lifei

AU - Van Berkel, Gary J.

AU - Kertesz, Vilmos

AU - Gan, Jinping

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AB - Herein, quantitation aspects of a fully automated autosampler/HPLC-MS/MS system applied for unattended droplet-based surface sampling of repaglinide dosed thin tissue sections with subsequent HPLC separation and mass spectrometric analysis of parent drug and various drug metabolites were studied. Major organs (brain, lung, liver, kidney and muscle) from whole-body thin tissue sections and corresponding organ homogenates prepared from repaglinide dosed mice were sampled by surface sampling and by bulk extraction, respectively, and analyzed by HPLC-MS/MS. A semi-quantitative agreement between data obtained by surface sampling and that by employing organ homogenate extraction was observed. Drug concentrations obtained by the two methods followed the same patterns for post-dose time points (0.25, 0.5, 1 and 2 h). Drug amounts determined in the specific tissues was typically higher when analyzing extracts from the organ homogenates. In addition, relative comparison of the levels of individual metabolites between the two analytical methods also revealed good semi-quantitative agreement.

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Quantitation of repaglinide and metabolites in mouse whole-body thin tissue sections using droplet-based liquid microjunction surface sampling-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry

Abstract

Funding

Keywords

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