Purification of sperm from high WBC semen samples using a spiral channel

Jiyoung Son, Bruce K. Gale, James M. Hotaling, Douglas T. Carrell

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

This study demonstrated the use of inertial microfluidic technology to separate sperm from white blood cells (WBC), the major contaminant of leukospermia semen samples, by flow focusing the sperm and WBCs. Fundamentals of inertial microfluidics were used to design the spiral channel. The fabricated spiral channel was verified in a series of experiments with WBC spiked semen samples. Concentration data and high speed streak images shows that sperm of high WBC semen sample can be significantly enriched by moderate sperm flow focusing and clear WBC flow focusing within a few minutes (∼5mins). These results suggest the promising potential for this technique to be used clinically.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages248-249
Number of pages2
ISBN (Electronic)9780979806490
StatePublished - 2016
Externally publishedYes
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: Oct 9 2016Oct 13 2016

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/TerritoryIreland
CityDublin
Period10/9/1610/13/16

Keywords

  • Inertial microfluidics
  • Sperm
  • Spiral channel
  • White blood cells (WBC)

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