Proteome analysis of the metal reducing microbe Shewanella oneidensis MR-1

N. C. VerBerkmoes; J. L. Bundy; L. Hauser; K. G. Asano; J. Razumovskaya; F. Larimer; R. L. Hettich; J. L. Stephenson

Proteome analysis of the metal reducing microbe Shewanella oneidensis MR-1

N. C. VerBerkmoes, J. L. Bundy, L. Hauser, K. G. Asano, J. Razumovskaya, F. Larimer, R. L. Hettich, J. L. Stephenson

Bioanalytical Mass Spectrometry

Research output: Contribution to conference › Paper › peer-review

Abstract

The techniques, LC-MS/MS were used for comprehensive microbial proteome characterization of Shewanella oneidensis MR-1, a metal reducing microbe. S. oneidensis cells were harvested in mid-log growth phase, disrupted by sonication, and fractioned into 2 membrane/insoluble fractions, a crude soluble fraction and a cleared soluble fraction. All the four fractions were digested with trypsin and analyzed by on-line 1D or 2D capillary chromatography coupled to an electrospray ion trap mass spectrometer operating in data dependent MS/MS mode. The method resulted in the identification of a significant number of new proteins that were being masked by the highly abundant ribosomal proteins.

Original language	English
Pages	61-62
Number of pages	2
State	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	06/2/02 → 06/6/02

Cite this

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title = "Proteome analysis of the metal reducing microbe Shewanella oneidensis MR-1",

abstract = "The techniques, LC-MS/MS were used for comprehensive microbial proteome characterization of Shewanella oneidensis MR-1, a metal reducing microbe. S. oneidensis cells were harvested in mid-log growth phase, disrupted by sonication, and fractioned into 2 membrane/insoluble fractions, a crude soluble fraction and a cleared soluble fraction. All the four fractions were digested with trypsin and analyzed by on-line 1D or 2D capillary chromatography coupled to an electrospray ion trap mass spectrometer operating in data dependent MS/MS mode. The method resulted in the identification of a significant number of new proteins that were being masked by the highly abundant ribosomal proteins.",

author = "VerBerkmoes, {N. C.} and Bundy, {J. L.} and L. Hauser and Asano, {K. G.} and J. Razumovskaya and F. Larimer and Hettich, {R. L.} and Stephenson, {J. L.}",

year = "2002",

language = "English",

pages = "61--62",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

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T1 - Proteome analysis of the metal reducing microbe Shewanella oneidensis MR-1

AU - VerBerkmoes, N. C.

AU - Bundy, J. L.

AU - Hauser, L.

AU - Asano, K. G.

AU - Razumovskaya, J.

AU - Larimer, F.

AU - Hettich, R. L.

AU - Stephenson, J. L.

PY - 2002

Y1 - 2002

N2 - The techniques, LC-MS/MS were used for comprehensive microbial proteome characterization of Shewanella oneidensis MR-1, a metal reducing microbe. S. oneidensis cells were harvested in mid-log growth phase, disrupted by sonication, and fractioned into 2 membrane/insoluble fractions, a crude soluble fraction and a cleared soluble fraction. All the four fractions were digested with trypsin and analyzed by on-line 1D or 2D capillary chromatography coupled to an electrospray ion trap mass spectrometer operating in data dependent MS/MS mode. The method resulted in the identification of a significant number of new proteins that were being masked by the highly abundant ribosomal proteins.

AB - The techniques, LC-MS/MS were used for comprehensive microbial proteome characterization of Shewanella oneidensis MR-1, a metal reducing microbe. S. oneidensis cells were harvested in mid-log growth phase, disrupted by sonication, and fractioned into 2 membrane/insoluble fractions, a crude soluble fraction and a cleared soluble fraction. All the four fractions were digested with trypsin and analyzed by on-line 1D or 2D capillary chromatography coupled to an electrospray ion trap mass spectrometer operating in data dependent MS/MS mode. The method resulted in the identification of a significant number of new proteins that were being masked by the highly abundant ribosomal proteins.

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M3 - Paper

AN - SCOPUS:2442644213

SP - 61

EP - 62

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Proteome analysis of the metal reducing microbe Shewanella oneidensis MR-1

Abstract

Conference

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