Protein loaded biodegradable microspheres based on PLGA-protein bioconjugates

Y. S. Nam; T. G. Park

doi:10.1080/026520499288816

Protein loaded biodegradable microspheres based on PLGA-protein bioconjugates

Y. S. Nam, T. G. Park

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Biodegradable poly(D,L-lactic-co-glycolic acid) (PLGA) was chemically conjugated to lysozyme, a model protein drug, by coupling a terminal carboxylic acid in PLGA with primary amine groups present in lysozyme. The conjugation was carried out in dimethylsulphoside (DMSO) by using carbodiimide as a coupling agent. The PLGA-lysozyme conjugate, dissolved in a co-solvent system of DMSO and methylene chloride, was directly formulated into microspheres by an oil-in-water (O/W) single emulsion solvent evaporation technique. Morphological characteristics of the resultant microspheres, loading efficiencies, and protein release behaviours with protein instability problems were investigated in comparison with those of the microspheres prepared by water-in-oil-water (W/O/W) double emulsion and O/W single emulsion techniques which employed PLGA with unconjugated lysozyme for the formulation.

Original language	English
Pages (from-to)	625-637
Number of pages	13
Journal	Journal of Microencapsulation
Volume	16
Issue number	5
DOIs	https://doi.org/10.1080/026520499288816
State	Published - Sep 1999
Externally published	Yes

Keywords

Conjugation
Microspheres
PLGA
Protein delivery
Protein stability

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10.1080/026520499288816

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title = "Protein loaded biodegradable microspheres based on PLGA-protein bioconjugates",

abstract = "Biodegradable poly(D,L-lactic-co-glycolic acid) (PLGA) was chemically conjugated to lysozyme, a model protein drug, by coupling a terminal carboxylic acid in PLGA with primary amine groups present in lysozyme. The conjugation was carried out in dimethylsulphoside (DMSO) by using carbodiimide as a coupling agent. The PLGA-lysozyme conjugate, dissolved in a co-solvent system of DMSO and methylene chloride, was directly formulated into microspheres by an oil-in-water (O/W) single emulsion solvent evaporation technique. Morphological characteristics of the resultant microspheres, loading efficiencies, and protein release behaviours with protein instability problems were investigated in comparison with those of the microspheres prepared by water-in-oil-water (W/O/W) double emulsion and O/W single emulsion techniques which employed PLGA with unconjugated lysozyme for the formulation.",

keywords = "Conjugation, Microspheres, PLGA, Protein delivery, Protein stability",

author = "Nam, {Y. S.} and Park, {T. G.}",

year = "1999",

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TY - JOUR

T1 - Protein loaded biodegradable microspheres based on PLGA-protein bioconjugates

AU - Nam, Y. S.

AU - Park, T. G.

PY - 1999/9

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N2 - Biodegradable poly(D,L-lactic-co-glycolic acid) (PLGA) was chemically conjugated to lysozyme, a model protein drug, by coupling a terminal carboxylic acid in PLGA with primary amine groups present in lysozyme. The conjugation was carried out in dimethylsulphoside (DMSO) by using carbodiimide as a coupling agent. The PLGA-lysozyme conjugate, dissolved in a co-solvent system of DMSO and methylene chloride, was directly formulated into microspheres by an oil-in-water (O/W) single emulsion solvent evaporation technique. Morphological characteristics of the resultant microspheres, loading efficiencies, and protein release behaviours with protein instability problems were investigated in comparison with those of the microspheres prepared by water-in-oil-water (W/O/W) double emulsion and O/W single emulsion techniques which employed PLGA with unconjugated lysozyme for the formulation.

AB - Biodegradable poly(D,L-lactic-co-glycolic acid) (PLGA) was chemically conjugated to lysozyme, a model protein drug, by coupling a terminal carboxylic acid in PLGA with primary amine groups present in lysozyme. The conjugation was carried out in dimethylsulphoside (DMSO) by using carbodiimide as a coupling agent. The PLGA-lysozyme conjugate, dissolved in a co-solvent system of DMSO and methylene chloride, was directly formulated into microspheres by an oil-in-water (O/W) single emulsion solvent evaporation technique. Morphological characteristics of the resultant microspheres, loading efficiencies, and protein release behaviours with protein instability problems were investigated in comparison with those of the microspheres prepared by water-in-oil-water (W/O/W) double emulsion and O/W single emulsion techniques which employed PLGA with unconjugated lysozyme for the formulation.

KW - Conjugation

KW - Microspheres

KW - PLGA

KW - Protein delivery

KW - Protein stability

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EP - 637

JO - Journal of Microencapsulation

JF - Journal of Microencapsulation

IS - 5

ER -

Protein loaded biodegradable microspheres based on PLGA-protein bioconjugates

Abstract

Keywords

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