Protein and solvent dynamics: How strongly are they coupled?

G. Caliskan; D. Mechtani; J. H. Roh; A. Kisliuk; A. P. Sokolov; S. Azzam; M. T. Cicerone; S. Lin-Gibson; I. Peral

doi:10.1063/1.1764491

Protein and solvent dynamics: How strongly are they coupled?

G. Caliskan, D. Mechtani, J. H. Roh, A. Kisliuk, A. P. Sokolov, S. Azzam, M. T. Cicerone, S. Lin-Gibson, I. Peral

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Abstract

The impact of various solvents on protein dynamics was investigated using Raman and neutron scattering spectra. Over the entire temperature range 100-350 K, the protein dynamics followed the dynamics of the solvents glycerol and trehalose. The behavior of the solvents was found sensitive to the protein's fast conformational fluctuations and low-frequency vibrations and their temperature variations. Glycerol acted as a biological cryopreservant, as shown by the results. The results also indicate that protein relaxation is stronger in solid trehalose than in liquid glycerol.

Original language	English
Pages (from-to)	1978-1983
Number of pages	6
Journal	Journal of Chemical Physics
Volume	121
Issue number	4
DOIs	https://doi.org/10.1063/1.1764491
State	Published - Jul 22 2004
Externally published	Yes

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title = "Protein and solvent dynamics: How strongly are they coupled?",

abstract = "The impact of various solvents on protein dynamics was investigated using Raman and neutron scattering spectra. Over the entire temperature range 100-350 K, the protein dynamics followed the dynamics of the solvents glycerol and trehalose. The behavior of the solvents was found sensitive to the protein's fast conformational fluctuations and low-frequency vibrations and their temperature variations. Glycerol acted as a biological cryopreservant, as shown by the results. The results also indicate that protein relaxation is stronger in solid trehalose than in liquid glycerol.",

author = "G. Caliskan and D. Mechtani and Roh, {J. H.} and A. Kisliuk and Sokolov, {A. P.} and S. Azzam and Cicerone, {M. T.} and S. Lin-Gibson and I. Peral",

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T1 - Protein and solvent dynamics

T2 - How strongly are they coupled?

AU - Caliskan, G.

AU - Mechtani, D.

AU - Roh, J. H.

AU - Kisliuk, A.

AU - Sokolov, A. P.

AU - Azzam, S.

AU - Cicerone, M. T.

AU - Lin-Gibson, S.

AU - Peral, I.

PY - 2004/7/22

Y1 - 2004/7/22

N2 - The impact of various solvents on protein dynamics was investigated using Raman and neutron scattering spectra. Over the entire temperature range 100-350 K, the protein dynamics followed the dynamics of the solvents glycerol and trehalose. The behavior of the solvents was found sensitive to the protein's fast conformational fluctuations and low-frequency vibrations and their temperature variations. Glycerol acted as a biological cryopreservant, as shown by the results. The results also indicate that protein relaxation is stronger in solid trehalose than in liquid glycerol.

AB - The impact of various solvents on protein dynamics was investigated using Raman and neutron scattering spectra. Over the entire temperature range 100-350 K, the protein dynamics followed the dynamics of the solvents glycerol and trehalose. The behavior of the solvents was found sensitive to the protein's fast conformational fluctuations and low-frequency vibrations and their temperature variations. Glycerol acted as a biological cryopreservant, as shown by the results. The results also indicate that protein relaxation is stronger in solid trehalose than in liquid glycerol.

UR - http://www.scopus.com/inward/record.url?scp=3543126540&partnerID=8YFLogxK

U2 - 10.1063/1.1764491

DO - 10.1063/1.1764491

M3 - Article

C2 - 15260750

AN - SCOPUS:3543126540

SN - 0021-9606

VL - 121

SP - 1978

EP - 1983

JO - Journal of Chemical Physics

JF - Journal of Chemical Physics

IS - 4

ER -

Protein and solvent dynamics: How strongly are they coupled?

Abstract

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