Properties of carbohydrate-metabolizing enzymes immobilized in sol-gel beads: Stabilization of invertase and β-glucosidase by Blue Dextran

Hugh O’neill; Catherine V. Angley; Isabelle Hemery; Barbara R. Evans; Sheng Dai; Jonathan Woodward

doi:10.1023/A:1015572020633

Properties of carbohydrate-metabolizing enzymes immobilized in sol-gel beads: Stabilization of invertase and β-glucosidase by Blue Dextran

Hugh O’neill, Catherine V. Angley, Isabelle Hemery, Barbara R. Evans, Sheng Dai, Jonathan Woodward

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

When immobilized in sol-gels, invertase (β-fructofuranosidase) from Candida utilis and β-glucosidase from Pyrococcus furiosus had activity recovery values of 30 and 28%, respectively. However, if Blue Dextran (0.04%) was included in the immobilization-reaction mixture, the respective recovery values increased to 63 and 52%. Glucose dehydrogenase from Thermoplasma acidophilum immobilized by the same method lost most of its activity and Blue Dextran had no effect on the recovery of activity during the immobilization procedure. The immobilized enzymes required treatment with glutaraldehyde in order to maintain their activity within the sol-gel matrix during continuous reaction with their respective substrates.

Original language	English
Article number	5086696
Pages (from-to)	783-790
Number of pages	8
Journal	Biotechnology Letters
Volume	24
Issue number	10
DOIs	https://doi.org/10.1023/A:1015572020633
State	Published - 2002

Funding

This work was supported by the Defense Advanced Research Projects Agency (DARPA). Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for the US Department of Energy under contract DE-AC05-00OR22725.

Funders	Funder number
US Department of Energy	DE-AC05-00OR22725
Defense Advanced Research Projects Agency
Oak Ridge National Laboratory

Keywords

Blue Dextran
Enzyme
Glutaraldehyde
Immobilization
Sol-gel

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abstract = "When immobilized in sol-gels, invertase (β-fructofuranosidase) from Candida utilis and β-glucosidase from Pyrococcus furiosus had activity recovery values of 30 and 28%, respectively. However, if Blue Dextran (0.04%) was included in the immobilization-reaction mixture, the respective recovery values increased to 63 and 52%. Glucose dehydrogenase from Thermoplasma acidophilum immobilized by the same method lost most of its activity and Blue Dextran had no effect on the recovery of activity during the immobilization procedure. The immobilized enzymes required treatment with glutaraldehyde in order to maintain their activity within the sol-gel matrix during continuous reaction with their respective substrates.",

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author = "Hugh O{\textquoteright}neill and Angley, {Catherine V.} and Isabelle Hemery and Evans, {Barbara R.} and Sheng Dai and Jonathan Woodward",

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T2 - Stabilization of invertase and β-glucosidase by Blue Dextran

AU - O’neill, Hugh

AU - Angley, Catherine V.

AU - Hemery, Isabelle

AU - Evans, Barbara R.

AU - Dai, Sheng

AU - Woodward, Jonathan

PY - 2002

Y1 - 2002

N2 - When immobilized in sol-gels, invertase (β-fructofuranosidase) from Candida utilis and β-glucosidase from Pyrococcus furiosus had activity recovery values of 30 and 28%, respectively. However, if Blue Dextran (0.04%) was included in the immobilization-reaction mixture, the respective recovery values increased to 63 and 52%. Glucose dehydrogenase from Thermoplasma acidophilum immobilized by the same method lost most of its activity and Blue Dextran had no effect on the recovery of activity during the immobilization procedure. The immobilized enzymes required treatment with glutaraldehyde in order to maintain their activity within the sol-gel matrix during continuous reaction with their respective substrates.

AB - When immobilized in sol-gels, invertase (β-fructofuranosidase) from Candida utilis and β-glucosidase from Pyrococcus furiosus had activity recovery values of 30 and 28%, respectively. However, if Blue Dextran (0.04%) was included in the immobilization-reaction mixture, the respective recovery values increased to 63 and 52%. Glucose dehydrogenase from Thermoplasma acidophilum immobilized by the same method lost most of its activity and Blue Dextran had no effect on the recovery of activity during the immobilization procedure. The immobilized enzymes required treatment with glutaraldehyde in order to maintain their activity within the sol-gel matrix during continuous reaction with their respective substrates.

KW - Blue Dextran

KW - Enzyme

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Properties of carbohydrate-metabolizing enzymes immobilized in sol-gel beads: Stabilization of invertase and β-glucosidase by Blue Dextran

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