Probing the relative orientation of molecules bound to DNA through controlled interference using second-harmonic generation

Benjamin Doughty; Yi Rao; Samuel W. Kazer; Sheldon J.J. Kwok; Nicholas J. Turro; Kenneth B. Eisenthal

doi:10.1073/pnas.1302554110

Probing the relative orientation of molecules bound to DNA through controlled interference using second-harmonic generation

Benjamin Doughty, Yi Rao, Samuel W. Kazer, Sheldon J.J. Kwok, Nicholas J. Turro, Kenneth B. Eisenthal

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

A method is described in which the interference of radiated secondharmonic electric fields generated by a pair of oriented molecules intercalated into double-stranded DNA is controlled and measured. The results show that the relative molecular orientation of the two molecules significantly changes the magnitude of the observed second-harmonic generation intensity, which is described by a simple model that accounts for the interferences of the radiated fields. The technique presented shows promise for future experiments investigating structural changes induced bythe formation of a DNA-biomolecule complex.

Original language	English
Pages (from-to)	5756-5758
Number of pages	3
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	110
Issue number	15
DOIs	https://doi.org/10.1073/pnas.1302554110
State	Published - Apr 9 2013
Externally published	Yes

Keywords

Alignment
Daunorubicin
Equilibrium binding
Nonlinear spectroscopy
Phase

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10.1073/pnas.1302554110

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title = "Probing the relative orientation of molecules bound to DNA through controlled interference using second-harmonic generation",

abstract = "A method is described in which the interference of radiated secondharmonic electric fields generated by a pair of oriented molecules intercalated into double-stranded DNA is controlled and measured. The results show that the relative molecular orientation of the two molecules significantly changes the magnitude of the observed second-harmonic generation intensity, which is described by a simple model that accounts for the interferences of the radiated fields. The technique presented shows promise for future experiments investigating structural changes induced bythe formation of a DNA-biomolecule complex.",

keywords = "Alignment, Daunorubicin, Equilibrium binding, Nonlinear spectroscopy, Phase",

author = "Benjamin Doughty and Yi Rao and Kazer, \{Samuel W.\} and Kwok, \{Sheldon J.J.\} and Turro, \{Nicholas J.\} and Eisenthal, \{Kenneth B.\}",

year = "2013",

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day = "9",

doi = "10.1073/pnas.1302554110",

language = "English",

volume = "110",

pages = "5756--5758",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

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Probing the relative orientation of molecules bound to DNA through controlled interference using second-harmonic generation. / Doughty, Benjamin; Rao, Yi; Kazer, Samuel W. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 15, 09.04.2013, p. 5756-5758.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Probing the relative orientation of molecules bound to DNA through controlled interference using second-harmonic generation

AU - Doughty, Benjamin

AU - Rao, Yi

AU - Kazer, Samuel W.

AU - Kwok, Sheldon J.J.

AU - Turro, Nicholas J.

AU - Eisenthal, Kenneth B.

PY - 2013/4/9

Y1 - 2013/4/9

N2 - A method is described in which the interference of radiated secondharmonic electric fields generated by a pair of oriented molecules intercalated into double-stranded DNA is controlled and measured. The results show that the relative molecular orientation of the two molecules significantly changes the magnitude of the observed second-harmonic generation intensity, which is described by a simple model that accounts for the interferences of the radiated fields. The technique presented shows promise for future experiments investigating structural changes induced bythe formation of a DNA-biomolecule complex.

AB - A method is described in which the interference of radiated secondharmonic electric fields generated by a pair of oriented molecules intercalated into double-stranded DNA is controlled and measured. The results show that the relative molecular orientation of the two molecules significantly changes the magnitude of the observed second-harmonic generation intensity, which is described by a simple model that accounts for the interferences of the radiated fields. The technique presented shows promise for future experiments investigating structural changes induced bythe formation of a DNA-biomolecule complex.

KW - Alignment

KW - Daunorubicin

KW - Equilibrium binding

KW - Nonlinear spectroscopy

KW - Phase

UR - https://www.scopus.com/pages/publications/84876046472

U2 - 10.1073/pnas.1302554110

DO - 10.1073/pnas.1302554110

M3 - Article

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AN - SCOPUS:84876046472

SN - 0027-8424

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 15

ER -

Probing the relative orientation of molecules bound to DNA through controlled interference using second-harmonic generation

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