Abstract
For antibiotics that target Gram-positive bacterial cell structures, optimizing their interaction with the cytoplasmic membrane is of paramount importance. Recent time-resolved second harmonic scattering (trSHS) experiments with living bacterial cells have shown that some amphiphilic small molecules display signals consistent with organization within the membrane environment. Such organization could arise, for example, from aggregation, solvent interactions, and/or environmental rigidity. To expand our study of this system, we turn to polarization-resolved SHS (pSHS). PSHS has previously been used with model membranes to extract information about the angular distribution of integrated small molecules. Here we apply pSHS, for the first time, to cells, specifically living Staphylococcus aureus. In doing so, we aim to address contributions ascribed to the organization of amphiphilic molecules in bacterial membranes.
Original language | English |
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Title of host publication | Ultrafast Nonlinear Imaging and Spectroscopy XII |
Editors | Zhiwen Liu, Demetri Psaltis, Kebin Shi |
Publisher | SPIE |
ISBN (Electronic) | 9781510679382 |
DOIs | |
State | Published - 2024 |
Externally published | Yes |
Event | Ultrafast Nonlinear Imaging and Spectroscopy XII 2024 - San Diego, United States Duration: Aug 18 2024 → Aug 20 2024 |
Publication series
Name | Proceedings of SPIE - The International Society for Optical Engineering |
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Volume | 13139 |
ISSN (Print) | 0277-786X |
ISSN (Electronic) | 1996-756X |
Conference
Conference | Ultrafast Nonlinear Imaging and Spectroscopy XII 2024 |
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Country/Territory | United States |
City | San Diego |
Period | 08/18/24 → 08/20/24 |
Funding
The authors would like to thank Pierre-Marie Gassin and Gaelle Gassin for useful conversations. This research was supported by the National Institute of General Medical Sciences (R35GM142928).
Keywords
- bacteria
- biological membranes
- nonlinear spectroscopy
- second harmonic scattering