Preliminary neutron and X-ray crystallographic studies of equine cyanomethemoglobin

A. Y. Kovalevsky, S. Zoe Fisher, Sean Seaver, Marat Mustyakimov, Narayanasami Sukumar, Paul Langan, Timothy C. Mueser, B. Leif Hanson

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Room-temperature and 100 K X-ray and room-temperature neutron diffraction data have been measured from equine cyanomethemoglobin to 1.7 Å resolution using a home source, to 1.6 Å resolution on NE-CAT at the Advanced Photon Source and to 2.0 Å resolution on the PCS at Los Alamos Neutron Science Center, respectively. The cyanomethemoglobin is in the R state and preliminary room-temperature electron and neutron scattering density maps clearly show the protonation states of potential Bohr groups. Interestingly, a water molecule that is in the vicinity of the heme group and coordinated to the distal histidine appears to be expelled from this site in the low-temperature structure.

Original languageEnglish
Pages (from-to)474-477
Number of pages4
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume66
Issue number4
DOIs
StatePublished - 2010
Externally publishedYes

Funding

FundersFunder number
National Institute of General Medical SciencesR01GM071939

    Keywords

    • Equine hemoglobin
    • Joint XN refinement
    • Protonation
    • R state
    • Time-of-flight neutron diffraction

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