Abstract
Poly(methylene blue) modified electrodes have been prepared by potential cycling from aqueous electrolyte solution containing methylene blue on glassy carbon substrates. They have been used successfully for the determination of haemoglobin via its electrooxidation at fixed potential using the batch injection analysis technique in which whole blood samples diluted with phosphate or acetate buffer are injected directly over the modified electrode immersed in inert electrolyte. Electrode response is fast, stable, and reproducible. For blood from healthy donors, a good linear correlation was found between the results from the electrochemical injection method and the conventional clinical spectrophotometric measurement after cyanidation. The blood from potentially ill donors showed deviations from the linear correlation suggesting that this may be a good method for detecting haematological problems. Copyright (C) 1999 Elsevier Science B.V.
| Original language | English |
|---|---|
| Pages (from-to) | 119-123 |
| Number of pages | 5 |
| Journal | Analytica Chimica Acta |
| Volume | 385 |
| Issue number | 1-3 |
| DOIs | |
| State | Published - Apr 5 1999 |
| Externally published | Yes |
Funding
The support of Instituto de Cooperação Cientı́fica e Tecnológica Internacional (ICCTI), Portugal (project 423/OFMB/SCIAE) and the National Technological Development Committee (OFMB), Hungary (project TéT P-1/96) in the framework of the Luso-Hungarian Cultural Agreement and National Scientific Research Fund, Hungary (OTKA-TO14928) is gratefully acknowledged.
| Funders | Funder number |
|---|---|
| Instituto de Cooperação Cientı́fica e Tecnológica Internacional | 423/OFMB/SCIAE |
| National Technological Development Committee | OTKA-TO14928, TéT P-1/96 |
Keywords
- Batch injection analysis
- Electrochemical sensor
- Haemoglobin
- Modified electrodes
- Poly(methylene blue)