TY - JOUR
T1 - Polyaniline label-based conductometric sensor for IgG detection
AU - Sergeyeva, Tatiana A.
AU - Lavrik, Nikolay V.
AU - Piletsky, Sergey A.
AU - Rachkov, Alexander E.
AU - El'skaya, Anna V.
PY - 1996/8
Y1 - 1996/8
N2 - A new method based on a biochemical binding system is developed for detecting the presence and measuring the concentration of a definite analyte in fluids. The conductometric scheme of detection has been employed for detection of the binding reaction of immunoglobulins. Two identical pairs of gold interdigitated electrodes deposited on the insulating solid support have been used as a detector. An electrically conducting polymer, namely polyaniline, has been proposed as a label for immunosensor development. A set of water-soluble forms of polyaniline with different molecular weights and oxidation levels have been prepared by chemical oxidation of aniline and characterized using gel-permeation chromatography. The acceptability of different kinds of polyaniline, and the selectivity and sensitivity of sensor response have been studied. The method of antibody detection in competitive electroimmunoassay has been developed. The lowest antigen concentration which may be detected in the competitive mode was found to be about 500 ng/ml. The results of impedance spectroscopy measurements obtained for different states of the electrodes are analyzed in order to clarify the mechanism of the sensor response.
AB - A new method based on a biochemical binding system is developed for detecting the presence and measuring the concentration of a definite analyte in fluids. The conductometric scheme of detection has been employed for detection of the binding reaction of immunoglobulins. Two identical pairs of gold interdigitated electrodes deposited on the insulating solid support have been used as a detector. An electrically conducting polymer, namely polyaniline, has been proposed as a label for immunosensor development. A set of water-soluble forms of polyaniline with different molecular weights and oxidation levels have been prepared by chemical oxidation of aniline and characterized using gel-permeation chromatography. The acceptability of different kinds of polyaniline, and the selectivity and sensitivity of sensor response have been studied. The method of antibody detection in competitive electroimmunoassay has been developed. The lowest antigen concentration which may be detected in the competitive mode was found to be about 500 ng/ml. The results of impedance spectroscopy measurements obtained for different states of the electrodes are analyzed in order to clarify the mechanism of the sensor response.
KW - Electroactive label
KW - Electrochemical immunoassay
KW - Immunosensor
KW - Polyaniline
UR - http://www.scopus.com/inward/record.url?scp=0042942209&partnerID=8YFLogxK
U2 - 10.1016/S0925-4005(97)80006-8
DO - 10.1016/S0925-4005(97)80006-8
M3 - Article
AN - SCOPUS:0042942209
SN - 0925-4005
VL - 34
SP - 283
EP - 288
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
IS - 1-3
ER -