Plant Promoters and Terminators for High-Precision Bioengineering

Emily G. Brooks; Estefania Elorriaga; Yang Liu; James R. Duduit; Guoliang Yuan; Chung Jui Tsai; Gerald A. Tuskan; Thomas G. Ranney; Xiaohan Yang; Wusheng Liu

doi:10.34133/bdr.0013

Plant Promoters and Terminators for High-Precision Bioengineering

Emily G. Brooks, Estefania Elorriaga, Yang Liu, James R. Duduit, Guoliang Yuan, Chung Jui Tsai, Gerald A. Tuskan, Thomas G. Ranney, Xiaohan Yang, Wusheng Liu

Research output: Contribution to journal › Review article › peer-review

16 Scopus citations

Abstract

High-precision bioengineering and synthetic biology require fine-tuning gene expression at both transcriptional and posttranscriptional levels. Gene transcription is tightly regulated by promoters and terminators. Promoters determine the timing, tissues and cells, and levels of the expression of genes. Terminators mediate transcription termination of genes and affect mRNA levels posttranscriptionally, e.g., the 3'-end processing, stability, translation efficiency, and nuclear to cytoplasmic export of mRNAs. The promoter and terminator combination affects gene expression. In the present article, we review the function and features of plant core promoters, proximal and distal promoters, and terminators, and their effects on and benchmarking strategies for regulating gene expression.

Original language	English
Article number	e0013
Journal	BioDesign Research
Volume	5
DOIs	https://doi.org/10.34133/bdr.0013
State	Published - 2023

Funding

Funding: The writing of this manuscript was supported by the USDA Floriculture and Nursery Research Initiative (FNRI) grant 8020–21000-071-23S, the USDA National Institute of Food and Agriculture (NIFA) Hatch project 02913, and the Center for Bioenergy Innovation (CBI), which is a U.S. Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. Oak Ridge National Laboratory is managed by UT-Battelle, LLC for the U.S. DOE under contract number DE-AC05-00OR22725. Author contributions: X.Y. and W.L. conceived the idea, and all authors wrote and approved the final manuscript. Competing interests: The authors declare that they have no competing interests.

Funders	Funder number
U.S. Department of Energy Bioenergy Research Center
USDA Floriculture and Nursery Research Initiative
U.S. Department of Energy	DE-AC05-00OR22725
National Institute of Food and Agriculture	02913
Office of Science
Biological and Environmental Research
Oak Ridge National Laboratory
Center for Bioenergy Innovation
Food and Nutrition Research Institute	8020–21000-071-23S

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title = "Plant Promoters and Terminators for High-Precision Bioengineering",

abstract = "High-precision bioengineering and synthetic biology require fine-tuning gene expression at both transcriptional and posttranscriptional levels. Gene transcription is tightly regulated by promoters and terminators. Promoters determine the timing, tissues and cells, and levels of the expression of genes. Terminators mediate transcription termination of genes and affect mRNA levels posttranscriptionally, e.g., the 3'-end processing, stability, translation efficiency, and nuclear to cytoplasmic export of mRNAs. The promoter and terminator combination affects gene expression. In the present article, we review the function and features of plant core promoters, proximal and distal promoters, and terminators, and their effects on and benchmarking strategies for regulating gene expression.",

author = "Brooks, {Emily G.} and Estefania Elorriaga and Yang Liu and Duduit, {James R.} and Guoliang Yuan and Tsai, {Chung Jui} and Tuskan, {Gerald A.} and Ranney, {Thomas G.} and Xiaohan Yang and Wusheng Liu",

note = "Publisher Copyright: {\textcopyright} 2023 Emily G. Brooks et al.",

year = "2023",

doi = "10.34133/bdr.0013",

language = "English",

volume = "5",

journal = "BioDesign Research",

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T1 - Plant Promoters and Terminators for High-Precision Bioengineering

AU - Brooks, Emily G.

AU - Elorriaga, Estefania

AU - Liu, Yang

AU - Duduit, James R.

AU - Yuan, Guoliang

AU - Tsai, Chung Jui

AU - Tuskan, Gerald A.

AU - Ranney, Thomas G.

AU - Yang, Xiaohan

AU - Liu, Wusheng

PY - 2023

Y1 - 2023

N2 - High-precision bioengineering and synthetic biology require fine-tuning gene expression at both transcriptional and posttranscriptional levels. Gene transcription is tightly regulated by promoters and terminators. Promoters determine the timing, tissues and cells, and levels of the expression of genes. Terminators mediate transcription termination of genes and affect mRNA levels posttranscriptionally, e.g., the 3'-end processing, stability, translation efficiency, and nuclear to cytoplasmic export of mRNAs. The promoter and terminator combination affects gene expression. In the present article, we review the function and features of plant core promoters, proximal and distal promoters, and terminators, and their effects on and benchmarking strategies for regulating gene expression.

AB - High-precision bioengineering and synthetic biology require fine-tuning gene expression at both transcriptional and posttranscriptional levels. Gene transcription is tightly regulated by promoters and terminators. Promoters determine the timing, tissues and cells, and levels of the expression of genes. Terminators mediate transcription termination of genes and affect mRNA levels posttranscriptionally, e.g., the 3'-end processing, stability, translation efficiency, and nuclear to cytoplasmic export of mRNAs. The promoter and terminator combination affects gene expression. In the present article, we review the function and features of plant core promoters, proximal and distal promoters, and terminators, and their effects on and benchmarking strategies for regulating gene expression.

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DO - 10.34133/bdr.0013

M3 - Review article

AN - SCOPUS:85171473794

SN - 2693-1257

VL - 5

JO - BioDesign Research

JF - BioDesign Research

M1 - e0013

ER -

Plant Promoters and Terminators for High-Precision Bioengineering

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