TY - JOUR
T1 - Phytoremediation and phytosensing of chemical contaminants, RDX and TNT
T2 - Identification of the required target genes
AU - Rao, Murali R.
AU - Halfhill, Matthew D.
AU - Abercrombie, Laura G.
AU - Ranjan, Priya
AU - Abercrombie, Jason M.
AU - Gouffon, Julia S.
AU - Saxton, Arnold M.
AU - Stewart, C. Neal
PY - 2009
Y1 - 2009
N2 - High explosives such as hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), and 2,4,6-trinitrotoluene (TNT) are important contaminants in the environment and phytoremediation has been viewed as a cost-effective abatement. There remains, however, an insufficient knowledge-base about how plants respond to explosives, especially in the steady state. Microarray analysis was conducted on Arabidopsis thaliana that were grown in Murashige and Skoog media containing steady-state levels of 0.5 mM RDX or 2.0 μM TNT to study the effect of these compounds on its transcriptional profile. Our results for both RDX and TNT were consistent with the existing theory for xenobiotic metabolism in plants. Among the genes that were differentially expressed included oxidoreductases, cytochrome P450s, transferases, transporters, and several unknown expressed proteins. We discuss the potential role of upregulated genes in plant metabolism, phytoremediation, and phytosensing. Phytosensing, the detection of field contamination using plants, is an end goal of this project.
AB - High explosives such as hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), and 2,4,6-trinitrotoluene (TNT) are important contaminants in the environment and phytoremediation has been viewed as a cost-effective abatement. There remains, however, an insufficient knowledge-base about how plants respond to explosives, especially in the steady state. Microarray analysis was conducted on Arabidopsis thaliana that were grown in Murashige and Skoog media containing steady-state levels of 0.5 mM RDX or 2.0 μM TNT to study the effect of these compounds on its transcriptional profile. Our results for both RDX and TNT were consistent with the existing theory for xenobiotic metabolism in plants. Among the genes that were differentially expressed included oxidoreductases, cytochrome P450s, transferases, transporters, and several unknown expressed proteins. We discuss the potential role of upregulated genes in plant metabolism, phytoremediation, and phytosensing. Phytosensing, the detection of field contamination using plants, is an end goal of this project.
KW - Explosives
KW - Microarrays
KW - Phytoremediation
KW - Phytosensing
KW - RDX
KW - TNT
UR - http://www.scopus.com/inward/record.url?scp=70350011935&partnerID=8YFLogxK
U2 - 10.1007/s10142-009-0125-z
DO - 10.1007/s10142-009-0125-z
M3 - Article
C2 - 19543758
AN - SCOPUS:70350011935
SN - 1438-793X
VL - 9
SP - 537
EP - 547
JO - Functional and Integrative Genomics
JF - Functional and Integrative Genomics
IS - 4
ER -