PEG-assisted DNA solubilization in organic solvents for preparing cytosol specifically degradable PEG/DNA nanogels

Hyejung Mok, Tae Gwan Park

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

DNA was dissolved in selected organic solvents in the presence of poly(ethylene glycol) (PEG). Nanoscale PEG/DNA complex (∼100 nm) was produced in dimethylsulfoxide (DMSO) phase. Using a thiol-functionalized six-arm branched PEG for DNA solubilization, the PEG/DNA nanocomplex was cross-linked through the formation of disulfide linkages between the thiol groups, resulting in the production of stable PEG/DNA nanogels in aqueous solution. DNA release from the nanogels could be modulated by changing the concentration of an external reducing agent. The released plasmid DNA from the nanogels maintained intact structural integrity and exhibited appreciable gene transfection efficiency. The PEG/DNA nanogels could be potentially applied for gene therapy including DNA vaccination.

Original languageEnglish
Pages (from-to)1369-1372
Number of pages4
JournalBioconjugate Chemistry
Volume17
Issue number6
DOIs
StatePublished - 2006
Externally publishedYes

Fingerprint

Dive into the research topics of 'PEG-assisted DNA solubilization in organic solvents for preparing cytosol specifically degradable PEG/DNA nanogels'. Together they form a unique fingerprint.

Cite this