Abstract
Crassulacean acid metabolism (CAM) is a specialized mode of photosynthesis that offers the potential to engineer improved water-use efficiency (WUE) and drought resilience in C3 plants while sustaining productivity in the hotter and drier climates that are predicted for much of the world. CAM species show an inverted pattern of stomatal opening and closing across the diel cycle, which conserves water and provides a means of maintaining growth in hot, water-limited environments. Recent genome sequencing of the constitutive model CAM species Kalanchoë fedtschenkoi provides a platform for elucidating the ensemble of proteins that link photosynthetic metabolism with stomatal movement, and that protect CAM plants from harsh environmental conditions. We describe a large-scale proteomics analysis to characterize and compare proteins, as well as diel changes in their abundance in guard cell-enriched epidermis and mesophyll cells from leaves of K. fedtschenkoi. Proteins implicated in processes that encompass respiration, the transport of water and CO2, stomatal regulation, and CAM biochemistry are highlighted and discussed. Diel rescheduling of guard cell starch turnover in K. fedtschenkoi compared with that observed in Arabidopsis is reported and tissue-specific localization in the epidermis and mesophyll of isozymes implicated in starch and malate turnover are discussed in line with the contrasting roles for these metabolites within the CAM mesophyll and stomatal complex. These data reveal the proteins and the biological processes enriched in each layer and provide key information for studies aiming to adapt plants to hot and dry environments by modifying leaf physiology for improved plant sustainability.
| Original language | English |
|---|---|
| Pages (from-to) | 869-888 |
| Number of pages | 20 |
| Journal | Plant Journal |
| Volume | 103 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jul 1 2020 |
Funding
This material is based upon work supported by the Department of Energy Office of Science Genomic Science Program under award number DE‐SC0008834. This research used resources of the Compute and Data Environment for Science (CADES) at the Oak Ridge National Laboratory. Oak Ridge National Laboratory is managed by UT‐Battelle, LLC for the US Department of Energy (under contract number DE‐AC05‐00OR22725). Additional funding was provided by Colciencias (NHC), BBSRC (DCT) and the Swiss National Science Foundation (SF) (grant no. 31003A‐166539/1 to DS). This material is based upon work supported by the Department of Energy Office of Science Genomic Science Program under award number DE-SC0008834. This research used resources of the Compute and Data Environment for Science (CADES) at the Oak Ridge National Laboratory. Oak Ridge National Laboratory is managed by UT-Battelle, LLC for the US Department of Energy (under contract number DE-AC05-00OR22725). Additional funding was provided by Colciencias (NHC), BBSRC (DCT) and the Swiss National Science Foundation (SF) (grant no. 31003A-166539/1 to DS). This manuscript has been authored by UT-Battelle, LLC under contract no. DE-AC05-00OR22725 with the US Department of Energy. The publisher, by accepting the article for publication, acknowledges that the United States Government retains a non-exclusive, paid-up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes. The Department of Energy will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan (http://energy.gov/downloads/doe-public-access-plan)
Keywords
- crassulacean acid metabolism
- epidermis
- guard cell
- proteomics
- starch metabolism
- Plant Proteins/metabolism
- Kalanchoe/metabolism
- Plant Epidermis/metabolism
- Organ Specificity
- Crassulacean Acid Metabolism
- Proteome/metabolism
- Mesophyll Cells/metabolism
- Photosynthesis
