Abstract
Resin 3D printing is attractive for the rapid fabrication of microscale cell culture devices, but common resin materials are unstable and cytotoxic under culture conditions. Strategies such as leaching or overcuring are insufficient to protect sensitive primary cells such as white blood cells. Here, we evaluated the effectiveness of using a parylene C coating of commercially available clear resins to prevent cytotoxic leaching, degradation of microfluidic devices, and absorption of small molecules. We found that parylene C significantly improved both the cytocompatibility with primary murine white blood cells and the material integrity of prints while maintaining the favorable optical qualities held by clear resins.
| Original language | English |
|---|---|
| Pages (from-to) | 3079-3083 |
| Number of pages | 5 |
| Journal | ACS Applied Bio Materials |
| Volume | 6 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 21 2023 |
| Externally published | Yes |
Funding
Research reported in this publication was funded by the National Institute of Biomedical Imaging and Bioengineering under Award U01EB029127, with cofunding from the National Center for Advancing Translational Sciences, and by the National Institute of Allergy and Infectious Diseases under Award R01AI131723. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. S.R.C. was supported, in part, by the 2021 Presidential Fellowship for Collaborative Neuroscience from the UVA Brain Institute and the 2022 Sidney M. Hecht Graduate Fellowship from UVA Chemistry. Parylene coating was performed at the Duke University Shared Materials Instrumentation Facility, a member of the North Carolina Research Triangle Nanotechnology Network, which is supported by the National Science Foundation (Award ECCS-2025064) as part of the National Nanotechnology Coordinated Infrastructure. The authors thank Kirk Bryson (Shared Materials Instrumentation Facility, Duke University) for his assistance and helpful correspondence. Profilometry was performed at UVA’s Nanoscale Materials Characterization Facility, and the authors acknowledge the assistance of Joseph Thompson and Djuro Raskovic (both at UVA) for their training and data acquisition. The authors thank Cameron Hawley (UVA) for administrative assistance and Brian O’Grady (Vanderbilt University) and Noo Li Jeon (Seoul National University) for helpful discussion regarding the merits of parylene coating.
Keywords
- additive manufacturing
- biocompatibility
- cell culture
- microfabrication
- microfluidics