Abstract
Background: Biochar ozonization was previously shown to dramatically increase its cation exchange capacity, thus improving its nutrient retention capacity. The potential soil application of ozonized biochar warrants the need for a toxicity study that investigates its effects on microorganisms. Results: In the study presented here, we found that the filtrates collected from ozonized pine 400 biochar and ozonized rogue biochar did not have any inhibitory effects on the soil environmental bacteria Pseudomonas putida, even at high dissolved organic carbon (DOC) concentrations of 300 ppm. However, the growth of Synechococcus elongatus PCC 7942 was inhibited by the ozonized biochar filtrates at DOC concentrations greater than 75 ppm. Further tests showed the presence of some potential inhibitory compounds (terephthalic acid and p-toluic acid) in the filtrate of non-ozonized pine 400 biochar; these compounds were greatly reduced upon wet-ozonization of the biochar material. Nutrient detection tests also showed that dry-ozonization of rogue biochar enhanced the availability of nitrate and phosphate in its filtrate, a property that may be desirable for soil application. Conclusion: Ozonized biochar substances can support soil environmental bacterium Pseudomonas putida growth, since ozonization detoxifies the potential inhibitory aromatic molecules. Graphical Abstract: [Figure not available: see fulltext.].
| Original language | English |
|---|---|
| Article number | 2 |
| Journal | Bioresources and Bioprocessing |
| Volume | 9 |
| Issue number | 1 |
| DOIs | |
| State | Published - Dec 2022 |
Funding
This research was supported in part by Prof. Lee's start-up research funds provided by the Department of Chemistry and Biochemistry, the College of Sciences, the Office of Research at ODU, and the ODU Research Foundation, and in part by the Center for Bioenergy Innovation funded by the Office of Biological and Environmental Research in the US Department of Energy Office of Science. We wish to thank for the support of this research in part by Prof. Lee's start-up research funds provided by the Department of Chemistry and Biochemistry, the College of Sciences, the Office of Research at ODU, and the ODU Research Foundation, and in part by the Center for Bioenergy Innovation funded by the Office of Biological and Environmental Research in the US Department of Energy Office of Science. The metabolite profiling research in the detection of potential bacterial inhibitory compounds was supported by the Center for Bioenergy Innovation funded by the Office of Biological and Environmental Research in the US Department of Energy Office of Science. Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for the United States Department of Energy under contract DE-AC05-00OR22725. We wish to thank for the support of this research in part by Prof. Lee's start-up research funds provided by the Department of Chemistry and Biochemistry, the College of Sciences, the Office of Research at ODU, and the ODU Research Foundation, and in part by the Center for Bioenergy Innovation funded by the Office of Biological and Environmental Research in the US Department of Energy Office of Science. The metabolite profiling research in the detection of potential bacterial inhibitory compounds was supported by the Center for Bioenergy Innovation funded by the Office of Biological and Environmental Research in the US Department of Energy Office of Science. Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for the United States Department of Energy under contract DE-AC05-00OR22725.
Keywords
- Bioassay
- Biological effects of ozonized biochar substances
- Dissolved organic carbon
- Ozonized biochar filtrate
- Pseudomonas putida