Ordered water around deuterated A-DNA by neutron fibre diffraction

L. H. Pope; M. W. Shotton; V. T. Forsyth; P. Langan; R. C. Denny; U. Giesen; M. T. Dauvergne; W. Fuller

doi:10.1016/S0921-4526(97)00814-4

Ordered water around deuterated A-DNA by neutron fibre diffraction

L. H. Pope, M. W. Shotton, V. T. Forsyth, P. Langan, R. C. Denny, U. Giesen, M. T. Dauvergne, W. Fuller

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A neutron fibre diffraction study of A-DNA hydration has been carried out using instrument D19 at the Institut Laue-Langevin, Grenoble. In contrast to earlier experiments, deuterated DNA was used in order to minimise incoherent scattering and sample absorption effects. Fourier analysis of data collected from the sample in a D₂O environment has revealed four distinct major groove ordered water sites. A network of ordered water involved in the bridging of successive O1 phosphate oxygen atoms along the double-helix backbone has been identified. Extensive hydration of the major groove base edges has been observed as a continuous core of density running down the helix axis. The other two hydration features are located in the centre of the major groove, one at the opening of the groove and the other located deep in the groove.

Original language	English
Pages (from-to)	1156-1158
Number of pages	3
Journal	Physica B: Physics of Condensed Matter
Volume	241-243
DOIs	https://doi.org/10.1016/S0921-4526(97)00814-4
State	Published - 1997
Externally published	Yes

Keywords

Deuterated DNA
Hydration
Neutron fibre diffraction

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10.1016/S0921-4526(97)00814-4

Cite this

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title = "Ordered water around deuterated A-DNA by neutron fibre diffraction",

abstract = "A neutron fibre diffraction study of A-DNA hydration has been carried out using instrument D19 at the Institut Laue-Langevin, Grenoble. In contrast to earlier experiments, deuterated DNA was used in order to minimise incoherent scattering and sample absorption effects. Fourier analysis of data collected from the sample in a D2O environment has revealed four distinct major groove ordered water sites. A network of ordered water involved in the bridging of successive O1 phosphate oxygen atoms along the double-helix backbone has been identified. Extensive hydration of the major groove base edges has been observed as a continuous core of density running down the helix axis. The other two hydration features are located in the centre of the major groove, one at the opening of the groove and the other located deep in the groove.",

keywords = "Deuterated DNA, Hydration, Neutron fibre diffraction",

author = "Pope, {L. H.} and Shotton, {M. W.} and Forsyth, {V. T.} and P. Langan and Denny, {R. C.} and U. Giesen and Dauvergne, {M. T.} and W. Fuller",

year = "1997",

doi = "10.1016/S0921-4526(97)00814-4",

language = "English",

volume = "241-243",

pages = "1156--1158",

journal = "Physica B: Physics of Condensed Matter",

issn = "0921-4526",

publisher = "Elsevier",

}

TY - JOUR

T1 - Ordered water around deuterated A-DNA by neutron fibre diffraction

AU - Pope, L. H.

AU - Shotton, M. W.

AU - Forsyth, V. T.

AU - Langan, P.

AU - Denny, R. C.

AU - Giesen, U.

AU - Dauvergne, M. T.

AU - Fuller, W.

PY - 1997

Y1 - 1997

N2 - A neutron fibre diffraction study of A-DNA hydration has been carried out using instrument D19 at the Institut Laue-Langevin, Grenoble. In contrast to earlier experiments, deuterated DNA was used in order to minimise incoherent scattering and sample absorption effects. Fourier analysis of data collected from the sample in a D2O environment has revealed four distinct major groove ordered water sites. A network of ordered water involved in the bridging of successive O1 phosphate oxygen atoms along the double-helix backbone has been identified. Extensive hydration of the major groove base edges has been observed as a continuous core of density running down the helix axis. The other two hydration features are located in the centre of the major groove, one at the opening of the groove and the other located deep in the groove.

AB - A neutron fibre diffraction study of A-DNA hydration has been carried out using instrument D19 at the Institut Laue-Langevin, Grenoble. In contrast to earlier experiments, deuterated DNA was used in order to minimise incoherent scattering and sample absorption effects. Fourier analysis of data collected from the sample in a D2O environment has revealed four distinct major groove ordered water sites. A network of ordered water involved in the bridging of successive O1 phosphate oxygen atoms along the double-helix backbone has been identified. Extensive hydration of the major groove base edges has been observed as a continuous core of density running down the helix axis. The other two hydration features are located in the centre of the major groove, one at the opening of the groove and the other located deep in the groove.

KW - Deuterated DNA

KW - Hydration

KW - Neutron fibre diffraction

UR - http://www.scopus.com/inward/record.url?scp=0001562994&partnerID=8YFLogxK

U2 - 10.1016/S0921-4526(97)00814-4

DO - 10.1016/S0921-4526(97)00814-4

M3 - Article

AN - SCOPUS:0001562994

SN - 0921-4526

VL - 241-243

SP - 1156

EP - 1158

JO - Physica B: Physics of Condensed Matter

JF - Physica B: Physics of Condensed Matter

ER -

Ordered water around deuterated A-DNA by neutron fibre diffraction

Abstract

Keywords

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