TY - GEN
T1 - OPTIMIZING STEREOLITHOGRAPHIC 3D-PRINTED MATERIALS FOR ON-CHIP PRIMARY IMMUNE CELL CULTURE
AU - Musgrove, Hannah
AU - Catterton, Megan
AU - Pompano, Rebecca R.
N1 - Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - The ability to analyze primary immune cells on-chip is imperative to individualizing drug testing. Stereolithographic (SLA) 3D printing is an attractive fabrication method for microscale devices for drug testing due to its high efficiency in producing complex chip designs. However, microfluidic SLA resins are not innately compatible for use with fragile, primary cell cultures due to cytotoxicity and print resolution. In this work, we optimize both printing and post-processing treatments for biocompatibility and print resolution of SLA resins while also characterizing material properties for feasibility of use with primary splenocyte and lymphocyte cell culture for observation with fluorescent microscopy.
AB - The ability to analyze primary immune cells on-chip is imperative to individualizing drug testing. Stereolithographic (SLA) 3D printing is an attractive fabrication method for microscale devices for drug testing due to its high efficiency in producing complex chip designs. However, microfluidic SLA resins are not innately compatible for use with fragile, primary cell cultures due to cytotoxicity and print resolution. In this work, we optimize both printing and post-processing treatments for biocompatibility and print resolution of SLA resins while also characterizing material properties for feasibility of use with primary splenocyte and lymphocyte cell culture for observation with fluorescent microscopy.
KW - cell culture
KW - microfluidic fabrication
KW - photo-polymerizable resin
KW - Stereolithographic 3D printing
UR - http://www.scopus.com/inward/record.url?scp=85114008272&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85114008272
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1423
EP - 1424
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -
