Optical melting as a tool for optimizing sequencing by hybridization (SBH) analysis of DNA

Mitchel J. Doktycz; K. Bruce Jacobson; K. L. Beattie; Robert S. Foote

doi:10.1117/12.206038

Optical melting as a tool for optimizing sequencing by hybridization (SBH) analysis of DNA

Mitchel J. Doktycz, K. Bruce Jacobson, K. L. Beattie, Robert S. Foote

Biosciences Division

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Sequencing by hybridization is a technique that relies on the specific hybridization properties of nucleic acids to derive sequence information. Hybridization properties are highly dependent on the DNA sequence and the solution environment. Identification of the optimal SBH conditions can be obtained by optical melting. By optical melting of 128 octamer pairs that have the appropriate choice of nucleic acid structures, a useful model of stability has been obtained which will aid in the design and implementation of SBH assays.

Original language	English
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Publisher	Society of Photo-Optical Instrumentation Engineers
Pages	30-34
Number of pages	5
Volume	2386
ISBN (Print)	0819417335, 9780819417336
DOIs	https://doi.org/10.1117/12.206038
State	Published - 1995
Event	Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics - San Jose, CA, USA Duration: Feb 8 1995 → Feb 10 1995

Conference

Conference	Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics
City	San Jose, CA, USA
Period	02/8/95 → 02/10/95

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10.1117/12.206038

Cite this

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title = "Optical melting as a tool for optimizing sequencing by hybridization (SBH) analysis of DNA",

abstract = "Sequencing by hybridization is a technique that relies on the specific hybridization properties of nucleic acids to derive sequence information. Hybridization properties are highly dependent on the DNA sequence and the solution environment. Identification of the optimal SBH conditions can be obtained by optical melting. By optical melting of 128 octamer pairs that have the appropriate choice of nucleic acid structures, a useful model of stability has been obtained which will aid in the design and implementation of SBH assays.",

author = "Doktycz, {Mitchel J.} and Jacobson, {K. Bruce} and Beattie, {K. L.} and Foote, {Robert S.}",

year = "1995",

doi = "10.1117/12.206038",

language = "English",

isbn = "0819417335",

volume = "2386",

pages = "30--34",

booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

publisher = "Society of Photo-Optical Instrumentation Engineers",

note = "Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics ; Conference date: 08-02-1995 Through 10-02-1995",

}

Doktycz, MJ, Jacobson, KB, Beattie, KL & Foote, RS 1995, Optical melting as a tool for optimizing sequencing by hybridization (SBH) analysis of DNA. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 2386, Society of Photo-Optical Instrumentation Engineers, pp. 30-34, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics, San Jose, CA, USA, 02/8/95. https://doi.org/10.1117/12.206038

Optical melting as a tool for optimizing sequencing by hybridization (SBH) analysis of DNA. / Doktycz, Mitchel J.; Jacobson, K. Bruce; Beattie, K. L. et al.
Proceedings of SPIE - The International Society for Optical Engineering. Vol. 2386 Society of Photo-Optical Instrumentation Engineers, 1995. p. 30-34.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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T1 - Optical melting as a tool for optimizing sequencing by hybridization (SBH) analysis of DNA

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AU - Jacobson, K. Bruce

AU - Beattie, K. L.

AU - Foote, Robert S.

PY - 1995

Y1 - 1995

N2 - Sequencing by hybridization is a technique that relies on the specific hybridization properties of nucleic acids to derive sequence information. Hybridization properties are highly dependent on the DNA sequence and the solution environment. Identification of the optimal SBH conditions can be obtained by optical melting. By optical melting of 128 octamer pairs that have the appropriate choice of nucleic acid structures, a useful model of stability has been obtained which will aid in the design and implementation of SBH assays.

AB - Sequencing by hybridization is a technique that relies on the specific hybridization properties of nucleic acids to derive sequence information. Hybridization properties are highly dependent on the DNA sequence and the solution environment. Identification of the optimal SBH conditions can be obtained by optical melting. By optical melting of 128 octamer pairs that have the appropriate choice of nucleic acid structures, a useful model of stability has been obtained which will aid in the design and implementation of SBH assays.

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M3 - Conference contribution

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SN - 9780819417336

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BT - Proceedings of SPIE - The International Society for Optical Engineering

PB - Society of Photo-Optical Instrumentation Engineers

T2 - Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics

Y2 - 8 February 1995 through 10 February 1995

ER -

Optical melting as a tool for optimizing sequencing by hybridization (SBH) analysis of DNA

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