Open conformation of ezrin bound to phosphatidylinositol 4,5-bisphosphate and to F-actin revealed by neutron scattering

Jayant James Jayasundar; Jeong Ho Ju; Lilin He; Dazhi Liu; Flora Meilleur; Jinkui Zhao; David J.E. Callaway; Zimei Bu

doi:10.1074/jbc.M112.380972

Open conformation of ezrin bound to phosphatidylinositol 4,5-bisphosphate and to F-actin revealed by neutron scattering

Jayant James Jayasundar
, Jeong Ho Ju
, Lilin He
, Dazhi Liu
, Flora Meilleur
, Jinkui Zhao
, David J.E. Callaway
, Zimei Bu

SANS & Spin Echo

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Background: The structure of activated ezrin is not known. Results: We have determined the conformation of activated ezrin upon binding to PIP2 and to F-actin. Conclusion: Activated ezrin forms more extensive contacts with F-actin than generally depicted. Significance: This study provides new insight into the mechanisms by which ezrin assembles signaling complexes at the membrane-cytoskeleton interface.

Original language	English
Pages (from-to)	37119-37133
Number of pages	15
Journal	Journal of Biological Chemistry
Volume	287
Issue number	44
DOIs	https://doi.org/10.1074/jbc.M112.380972
State	Published - Oct 26 2012

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title = "Open conformation of ezrin bound to phosphatidylinositol 4,5-bisphosphate and to F-actin revealed by neutron scattering",

abstract = "Background: The structure of activated ezrin is not known. Results: We have determined the conformation of activated ezrin upon binding to PIP2 and to F-actin. Conclusion: Activated ezrin forms more extensive contacts with F-actin than generally depicted. Significance: This study provides new insight into the mechanisms by which ezrin assembles signaling complexes at the membrane-cytoskeleton interface.",

author = "Jayasundar, \{Jayant James\} and Ju, \{Jeong Ho\} and Lilin He and Dazhi Liu and Flora Meilleur and Jinkui Zhao and Callaway, \{David J.E.\} and Zimei Bu",

year = "2012",

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doi = "10.1074/jbc.M112.380972",

language = "English",

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journal = "Journal of Biological Chemistry",

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T1 - Open conformation of ezrin bound to phosphatidylinositol 4,5-bisphosphate and to F-actin revealed by neutron scattering

AU - Jayasundar, Jayant James

AU - Ju, Jeong Ho

AU - He, Lilin

AU - Liu, Dazhi

AU - Meilleur, Flora

AU - Zhao, Jinkui

AU - Callaway, David J.E.

AU - Bu, Zimei

PY - 2012/10/26

Y1 - 2012/10/26

N2 - Background: The structure of activated ezrin is not known. Results: We have determined the conformation of activated ezrin upon binding to PIP2 and to F-actin. Conclusion: Activated ezrin forms more extensive contacts with F-actin than generally depicted. Significance: This study provides new insight into the mechanisms by which ezrin assembles signaling complexes at the membrane-cytoskeleton interface.

AB - Background: The structure of activated ezrin is not known. Results: We have determined the conformation of activated ezrin upon binding to PIP2 and to F-actin. Conclusion: Activated ezrin forms more extensive contacts with F-actin than generally depicted. Significance: This study provides new insight into the mechanisms by which ezrin assembles signaling complexes at the membrane-cytoskeleton interface.

UR - https://www.scopus.com/pages/publications/84868262769

U2 - 10.1074/jbc.M112.380972

DO - 10.1074/jbc.M112.380972

M3 - Article

C2 - 22927432

AN - SCOPUS:84868262769

SN - 0021-9258

VL - 287

SP - 37119

EP - 37133

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 44

ER -

Open conformation of ezrin bound to phosphatidylinositol 4,5-bisphosphate and to F-actin revealed by neutron scattering

Abstract

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