One- and two-dimensional LC/MS/MS analysis of Arabidopsis thaliana proteome

Nathan C. VerBerkmoes; Robert L. Hettich; Barry D. Bruce; Reno Nguyen; Theresa L. Savage

One- and two-dimensional LC/MS/MS analysis of Arabidopsis thaliana proteome

Nathan C. VerBerkmoes, Robert L. Hettich, Barry D. Bruce, Reno Nguyen, Theresa L. Savage

Bioanalytical Mass Spectrometry

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Proteome analysis requires robust separations and rapid protein identification. Currently, two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) followed by mass spectrometry (MS) analysis is the gold standard. Recently there has been much interest in developing non-gel-based separation techniques for proteome analysis. This application note compares a 1-D and a 2-D HPLC separation coupled to MS for proteome analysis and finds that the 2-D method identifies 30% more proteins.

Original language	English
Pages (from-to)	10-11
Number of pages	2
Journal	LC-GC North America
Volume	20
Issue number	8 SUPPL.
State	Published - Aug 1 2002

Cite this

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abstract = "Proteome analysis requires robust separations and rapid protein identification. Currently, two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) followed by mass spectrometry (MS) analysis is the gold standard. Recently there has been much interest in developing non-gel-based separation techniques for proteome analysis. This application note compares a 1-D and a 2-D HPLC separation coupled to MS for proteome analysis and finds that the 2-D method identifies 30\% more proteins.",

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AU - VerBerkmoes, Nathan C.

AU - Hettich, Robert L.

AU - Bruce, Barry D.

AU - Nguyen, Reno

AU - Savage, Theresa L.

PY - 2002/8/1

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N2 - Proteome analysis requires robust separations and rapid protein identification. Currently, two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) followed by mass spectrometry (MS) analysis is the gold standard. Recently there has been much interest in developing non-gel-based separation techniques for proteome analysis. This application note compares a 1-D and a 2-D HPLC separation coupled to MS for proteome analysis and finds that the 2-D method identifies 30% more proteins.

AB - Proteome analysis requires robust separations and rapid protein identification. Currently, two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) followed by mass spectrometry (MS) analysis is the gold standard. Recently there has been much interest in developing non-gel-based separation techniques for proteome analysis. This application note compares a 1-D and a 2-D HPLC separation coupled to MS for proteome analysis and finds that the 2-D method identifies 30% more proteins.

UR - https://www.scopus.com/pages/publications/0036705193

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AN - SCOPUS:0036705193

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JO - LC-GC North America

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IS - 8 SUPPL.

ER -

One- and two-dimensional LC/MS/MS analysis of Arabidopsis thaliana proteome

Abstract

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