One- and two-dimensional LC/MS/MS analysis of Arabidopsis thaliana proteome

Nathan C. VerBerkmoes; Robert L. Hettich; Barry D. Bruce; Reno Nguyen; Theresa L. Savage

One- and two-dimensional LC/MS/MS analysis of Arabidopsis thaliana proteome

Nathan C. VerBerkmoes, Robert L. Hettich, Barry D. Bruce, Reno Nguyen, Theresa L. Savage

Bioanalytical Mass Spectrometry

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Proteome analysis requires robust separations and rapid protein identification. Currently, two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) followed by mass spectrometry (MS) analysis is the gold standard. Recently there has been much interest in developing non-gel-based separation techniques for proteome analysis. This application note compares a 1-D and a 2-D HPLC separation coupled to MS for proteome analysis and finds that the 2-D method identifies 30% more proteins.

Original language	English
Pages (from-to)	10-11
Number of pages	2
Journal	LC-GC North America
Volume	20
Issue number	8 SUPPL.
State	Published - Aug 1 2002

Cite this

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AU - Savage, Theresa L.

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AB - Proteome analysis requires robust separations and rapid protein identification. Currently, two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) followed by mass spectrometry (MS) analysis is the gold standard. Recently there has been much interest in developing non-gel-based separation techniques for proteome analysis. This application note compares a 1-D and a 2-D HPLC separation coupled to MS for proteome analysis and finds that the 2-D method identifies 30% more proteins.

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One- and two-dimensional LC/MS/MS analysis of Arabidopsis thaliana proteome

Abstract

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