On the Use of Shape-Constrained Splines for Biokinetic Process Modeling

Identification of mathematical models is an important task for the design and the optimization of biokinetic processes. Monod or Tessier growth-rate models are often chosen by default, although these models are not able to represent the dynamics of all bacterial growth processes. This imperfect representation then affects the quality of the model prediction. This paper introduces an alternative approach, which is based on constraints such as monotonicity and concavity and the use of shape-constrained spline functions, to describe the substrate affinity with high parametric flexibility. This way, the difficult task of searching through potentially incomplete rate-model libraries can be circumvented. A simulated case study is used to illustrate the superiority of the proposed method to represent non-ideal growth conditions, where neither Monod nor Tessier kinetics offer a good approximation.