Oligomerization state and pigment binding strength of the peridinin-Chl a-protein

Jing Jiang; Hao Zhang; Xun Lu; Yue Lu; Matthew J. Cuneo; Hugh M. O'Neill; Volker Urban; Cynthia S. Lo; Robert E. Blankenship

doi:10.1016/j.febslet.2015.07.039

Oligomerization state and pigment binding strength of the peridinin-Chl a-protein

Jing Jiang, Hao Zhang, Xun Lu, Yue Lu, Matthew J. Cuneo, Hugh M. O'Neill, Volker Urban, Cynthia S. Lo, Robert E. Blankenship

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The peridinin-chlorophyll a-protein (PCP) is one of the major light harvesting complexes (LHCs) in photosynthetic dinoflagellates. We analyzed the oligomeric state of PCP isolated from the dinoflagellate Symbiodinium, which has received increasing attention in recent years because of its role in coral bleaching. Size-exclusion chromatography (SEC) and small angle neutron scattering (SANS) analysis indicated PCP exists as monomers. Native mass spectrometry (native MS) demonstrated two oligomeric states of PCP, with the monomeric PCP being dominant. The trimerization may not be necessary for PCP to function as a light-harvesting complex.

Original language	English
Pages (from-to)	2713-2719
Number of pages	7
Journal	FEBS Letters
Volume	589
Issue number	19
DOIs	https://doi.org/10.1016/j.febslet.2015.07.039
State	Published - Sep 14 2015

Bibliographical note

Publisher Copyright:
© 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Keywords

Chlorophyll
Dinoflagellate
Light-harvesting
Native mass spectrometry
Peridinin
Photosynthesis
Symbiodinium

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10.1016/j.febslet.2015.07.039

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title = "Oligomerization state and pigment binding strength of the peridinin-Chl a-protein",

abstract = "The peridinin-chlorophyll a-protein (PCP) is one of the major light harvesting complexes (LHCs) in photosynthetic dinoflagellates. We analyzed the oligomeric state of PCP isolated from the dinoflagellate Symbiodinium, which has received increasing attention in recent years because of its role in coral bleaching. Size-exclusion chromatography (SEC) and small angle neutron scattering (SANS) analysis indicated PCP exists as monomers. Native mass spectrometry (native MS) demonstrated two oligomeric states of PCP, with the monomeric PCP being dominant. The trimerization may not be necessary for PCP to function as a light-harvesting complex.",

keywords = "Chlorophyll, Dinoflagellate, Light-harvesting, Native mass spectrometry, Peridinin, Photosynthesis, Symbiodinium",

author = "Jing Jiang and Hao Zhang and Xun Lu and Yue Lu and Cuneo, {Matthew J.} and O'Neill, {Hugh M.} and Volker Urban and Lo, {Cynthia S.} and Blankenship, {Robert E.}",

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doi = "10.1016/j.febslet.2015.07.039",

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T1 - Oligomerization state and pigment binding strength of the peridinin-Chl a-protein

AU - Jiang, Jing

AU - Zhang, Hao

AU - Lu, Xun

AU - Lu, Yue

AU - Cuneo, Matthew J.

AU - O'Neill, Hugh M.

AU - Urban, Volker

AU - Lo, Cynthia S.

AU - Blankenship, Robert E.

PY - 2015/9/14

Y1 - 2015/9/14

N2 - The peridinin-chlorophyll a-protein (PCP) is one of the major light harvesting complexes (LHCs) in photosynthetic dinoflagellates. We analyzed the oligomeric state of PCP isolated from the dinoflagellate Symbiodinium, which has received increasing attention in recent years because of its role in coral bleaching. Size-exclusion chromatography (SEC) and small angle neutron scattering (SANS) analysis indicated PCP exists as monomers. Native mass spectrometry (native MS) demonstrated two oligomeric states of PCP, with the monomeric PCP being dominant. The trimerization may not be necessary for PCP to function as a light-harvesting complex.

AB - The peridinin-chlorophyll a-protein (PCP) is one of the major light harvesting complexes (LHCs) in photosynthetic dinoflagellates. We analyzed the oligomeric state of PCP isolated from the dinoflagellate Symbiodinium, which has received increasing attention in recent years because of its role in coral bleaching. Size-exclusion chromatography (SEC) and small angle neutron scattering (SANS) analysis indicated PCP exists as monomers. Native mass spectrometry (native MS) demonstrated two oligomeric states of PCP, with the monomeric PCP being dominant. The trimerization may not be necessary for PCP to function as a light-harvesting complex.

KW - Chlorophyll

KW - Dinoflagellate

KW - Light-harvesting

KW - Native mass spectrometry

KW - Peridinin

KW - Photosynthesis

KW - Symbiodinium

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U2 - 10.1016/j.febslet.2015.07.039

DO - 10.1016/j.febslet.2015.07.039

M3 - Article

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AN - SCOPUS:84941426406

SN - 0014-5793

VL - 589

SP - 2713

EP - 2719

JO - FEBS Letters

JF - FEBS Letters

IS - 19

ER -

Oligomerization state and pigment binding strength of the peridinin-Chl a-protein

Abstract

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Keywords

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