TY - JOUR
T1 - Novel Liposome-Based Surface-Enhanced Raman Spectroscopy (SERS) Substrate
AU - Lum, William
AU - Bruzas, Ian
AU - Gorunmez, Zohre
AU - Unser, Sarah
AU - Beck, Thomas
AU - Sagle, Laura
N1 - Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/6/15
Y1 - 2017/6/15
N2 - Although great strides have been made in recent years toward making highly enhancing surface-enhanced Raman spectroscopy (SERS) substrates, the biological compatibility of such substrates remains a crucial problem. To address this issue, liposome-based SERS substrates have been constructed in which the biological probe molecule is encapsulated inside the aqueous liposome compartment, and metallic elements are assembled using the liposome as a scaffold. Therefore, the probe molecule is not in contact with the metallic surfaces. Herein we report our initial characterization of these novel nanoparticle-on-mirror substrates, both experimentally and theoretically, using finite-difference time-domain calculations. The substrates are shown to be structurally stable to laser irradiation, the liposome compartment does not rise above 45 °C, and they exhibit an analytical enhancement factor of 8 × 106 for crystal violet encapsulated in 38 liposomes sandwiched between a 40 nm planar gold mirror and 80 nm gold colloid.
AB - Although great strides have been made in recent years toward making highly enhancing surface-enhanced Raman spectroscopy (SERS) substrates, the biological compatibility of such substrates remains a crucial problem. To address this issue, liposome-based SERS substrates have been constructed in which the biological probe molecule is encapsulated inside the aqueous liposome compartment, and metallic elements are assembled using the liposome as a scaffold. Therefore, the probe molecule is not in contact with the metallic surfaces. Herein we report our initial characterization of these novel nanoparticle-on-mirror substrates, both experimentally and theoretically, using finite-difference time-domain calculations. The substrates are shown to be structurally stable to laser irradiation, the liposome compartment does not rise above 45 °C, and they exhibit an analytical enhancement factor of 8 × 106 for crystal violet encapsulated in 38 liposomes sandwiched between a 40 nm planar gold mirror and 80 nm gold colloid.
UR - http://www.scopus.com/inward/record.url?scp=85020798531&partnerID=8YFLogxK
U2 - 10.1021/acs.jpclett.7b00694
DO - 10.1021/acs.jpclett.7b00694
M3 - Article
C2 - 28535675
AN - SCOPUS:85020798531
SN - 1948-7185
VL - 8
SP - 2639
EP - 2646
JO - Journal of Physical Chemistry Letters
JF - Journal of Physical Chemistry Letters
IS - 12
ER -