@article{5343301dba8e4564a1455bebb11055f1,
title = "Neutron scattering techniques and applications in structural biology",
abstract = "Neutron scattering is exquisitely sensitive to the position, concentration, and dynamics of hydrogen atoms in materials and is a powerful tool for the characterization of structurefunction and interfacial relationships in biological systems. Modern neutron scattering facilities offer access to a sophisticated, nondestructive suite of instruments for biophysical characterization that provides spatial and dynamic information spanning from {\AA}ngstroms to microns and from picoseconds to microseconds, respectively. Applications in structural biology range from the atomic-resolution analysis of individual hydrogen atoms in enzymes through to meso- and macro-scale analysis of complex biological structures, membranes, and assemblies. The large difference in neutron scattering length between hydrogen and deuterium allows contrast variation experiments to be performed and enables H/D isotopic labeling to be used for selective and systematic analysis of the local structure, dynamics, and interactions of multi-component systems. This overview describes the available techniques and summarizes their practical application to the study of biomolecular systems.",
keywords = "Bio-macromolecule structure, Deuteration, Diffraction, Membranes, Neutron scattering, Protein dynamics, Quasi-elastic, Reflectometry, SANS",
author = "Ankner, {John F.} and Heller, {William T.} and Herwig, {Kenneth W.} and Flora Meilleur and Myles, {Dean A.A.}",
year = "2013",
doi = "10.1002/0471140864.ps1716s72",
language = "English",
journal = "Current Protocols in Protein Science",
issn = "1934-3655",
number = "SUPPL.72",
}