Neutron fibre diffraction studies of DNA hydration

P. Langan; V. T. Forsyth; A. Mahendrasingam; U. Giesen; M. Th Dauvergne; S. A. Mason; C. C. Wilson; W. Fuller

doi:10.1016/0921-4526(95)00279-I

Neutron fibre diffraction studies of DNA hydration

P. Langan, V. T. Forsyth, A. Mahendrasingam, U. Giesen, M. Th Dauvergne, S. A. Mason, C. C. Wilson, W. Fuller

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Techniques for recording and analysing fibre diffraction data from DNA using the instrument D19 at the Institut Laue-Langevin Reactor Source and the instrument SXD at the Rutherford Appleton Laboratory ISIS Spallation Source are described and compared. Data recorded from a specially prepared sample of deuterated DNA in the A conformation using SXD are compared with previously analysed data from a normally hydrogenated DNA in the same conformation recorded on D19. A preliminary Fourier difference analysis of these two datasets has allowed their consistency to be established.

Original language	English
Pages (from-to)	783-785
Number of pages	3
Journal	Physica B: Physics of Condensed Matter
Volume	213-214
Issue number	C
DOIs	https://doi.org/10.1016/0921-4526(95)00279-I
State	Published - Aug 1 1995
Externally published	Yes

Funding

We thank the SERC for support through GR/F/83501 and GR/H/67959, to the ILL and ISIS for allocations of beam time, to EMBO for a fellowship to VTF and to EMBL, Grenoble for provision of deuterated cells and facilities for extraction and purification of DNA. We would also like to thank Dr. R. Leberman for assistance and advice during the period of the EMBO fellowship.

Funders	Funder number
ILL
ISIS
Connecticut State Emergency Response Commission	GR/F/83501, GR/H/67959
EMBO

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abstract = "Techniques for recording and analysing fibre diffraction data from DNA using the instrument D19 at the Institut Laue-Langevin Reactor Source and the instrument SXD at the Rutherford Appleton Laboratory ISIS Spallation Source are described and compared. Data recorded from a specially prepared sample of deuterated DNA in the A conformation using SXD are compared with previously analysed data from a normally hydrogenated DNA in the same conformation recorded on D19. A preliminary Fourier difference analysis of these two datasets has allowed their consistency to be established.",

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doi = "10.1016/0921-4526(95)00279-I",

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T1 - Neutron fibre diffraction studies of DNA hydration

AU - Langan, P.

AU - Forsyth, V. T.

AU - Mahendrasingam, A.

AU - Giesen, U.

AU - Dauvergne, M. Th

AU - Mason, S. A.

AU - Wilson, C. C.

AU - Fuller, W.

PY - 1995/8/1

Y1 - 1995/8/1

N2 - Techniques for recording and analysing fibre diffraction data from DNA using the instrument D19 at the Institut Laue-Langevin Reactor Source and the instrument SXD at the Rutherford Appleton Laboratory ISIS Spallation Source are described and compared. Data recorded from a specially prepared sample of deuterated DNA in the A conformation using SXD are compared with previously analysed data from a normally hydrogenated DNA in the same conformation recorded on D19. A preliminary Fourier difference analysis of these two datasets has allowed their consistency to be established.

AB - Techniques for recording and analysing fibre diffraction data from DNA using the instrument D19 at the Institut Laue-Langevin Reactor Source and the instrument SXD at the Rutherford Appleton Laboratory ISIS Spallation Source are described and compared. Data recorded from a specially prepared sample of deuterated DNA in the A conformation using SXD are compared with previously analysed data from a normally hydrogenated DNA in the same conformation recorded on D19. A preliminary Fourier difference analysis of these two datasets has allowed their consistency to be established.

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U2 - 10.1016/0921-4526(95)00279-I

DO - 10.1016/0921-4526(95)00279-I

M3 - Article

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JO - Physica B: Physics of Condensed Matter

JF - Physica B: Physics of Condensed Matter

IS - C

ER -

Neutron fibre diffraction studies of DNA hydration

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