Neutron diffraction from aligned stacks of lipid bilayers using the WAND instrument

Drew Marquardt, Matthias D. Frontzek, Yu Zhao, Bryan C. Chakoumakos, John Katsaras

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Neutron diffraction from aligned stacks of lipid bilayers is examined using the Wide-Angle Neutron Diffractometer (WAND), located at the High Flux Isotope Reactor, Oak Ridge, Tennessee, USA. Data were collected at different levels of hydration and neutron contrast by varying the relative humidity (RH) and H2O/D2O ratio from multi-bilayers of dioleoylphosphatidylcholine and sunflower phosphatidylcholine extract aligned on single-crystal silicon substrates. This work highlights the capabilites of a newly fabricated sample hydration cell, which allows the lipid bilayers to be hydrated with varying H/D ratios from the RH generated by saturated salt solutions, and also demonstrates WAND's capability as an instrument suitable for the study of aligned lipid multi-bilayers.

Original languageEnglish
Pages (from-to)235-241
Number of pages7
JournalJournal of Applied Crystallography
Volume51
Issue number2
DOIs
StatePublished - Apr 2018

Funding

JK is supported through the Scientific User Facilities Division of the US Department of Energy (DOE), Office of Basic Energy Sciences (BES), under contract No. DE-AC05-00OR2275. This research used resources at the High Flux Isotope Reactor (HFIR), a DOE Office of Science User Facility operated by the Oak Ridge National Laboratory (ORNL). Some of this work was performed at ORNL’s Center for Structural Molecular Biology (CSMB) sponsored by the Office of Biological and Environmental Research (BER), US DOE.

Keywords

  • deuteration
  • lipid bilayers
  • neutron diffraction
  • relative humidity

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