TY - JOUR
T1 - Neutron diffraction analysis of Pseudomonas aeruginosa peptidyl-tRNA hydrolase 1
AU - McFeeters, Hana
AU - Vandavasi, Venu Gopal
AU - Weiss, Kevin L.
AU - Coates, Leighton
AU - McFeeters, Robert L.
N1 - Publisher Copyright:
© 2016 International Union of Crystallography.
PY - 2016
Y1 - 2016
N2 - Perdeuterated peptidyl-tRNA hydrolase 1 from Pseudomonas aeruginosa was crystallized for structural analysis using neutron diffraction. Crystals of perdeuterated protein were grown to 0.15 mm3 in size using batch crystallization in 22.5% polyethylene glycol 4000, 100 mM Tris pH 7.5, 10%(v/v) isopropyl alcohol with a 20-molar excess of trilysine as an additive. Neutron diffraction data were collected from a crystal at room temperature using the MaNDi single-crystal diffractometer at Oak Ridge National Laboratory.
AB - Perdeuterated peptidyl-tRNA hydrolase 1 from Pseudomonas aeruginosa was crystallized for structural analysis using neutron diffraction. Crystals of perdeuterated protein were grown to 0.15 mm3 in size using batch crystallization in 22.5% polyethylene glycol 4000, 100 mM Tris pH 7.5, 10%(v/v) isopropyl alcohol with a 20-molar excess of trilysine as an additive. Neutron diffraction data were collected from a crystal at room temperature using the MaNDi single-crystal diffractometer at Oak Ridge National Laboratory.
KW - Pseudomonas aeruginosa
KW - antibiotic resistance
KW - neutron diffraction
KW - peptidyl-tRNA hydrolase 1
KW - perdeuteration
UR - http://www.scopus.com/inward/record.url?scp=84959909871&partnerID=8YFLogxK
U2 - 10.1107/S2053230X16001813
DO - 10.1107/S2053230X16001813
M3 - Article
C2 - 26919526
AN - SCOPUS:84959909871
SN - 2053-230X
VL - 72
SP - 220
EP - 223
JO - Acta Crystallographica Section F:Structural Biology Communications
JF - Acta Crystallographica Section F:Structural Biology Communications
ER -