Neutron and X-ray analysis of the Fenna-Matthews-Olson photosynthetic antenna complex from Prosthecochloris aestuarii

Xun Lu; Brinda Selvaraj; Sudipa Ghimire-Rijal; Gregory S. Orf; Flora Meilleur; Robert E. Blankenship; Matthew J. Cuneo; Dean A.A. Myles

doi:10.1107/S2053230X19000724

Neutron and X-ray analysis of the Fenna-Matthews-Olson photosynthetic antenna complex from Prosthecochloris aestuarii

Xun Lu, Brinda Selvaraj, Sudipa Ghimire-Rijal, Gregory S. Orf, Flora Meilleur, Robert E. Blankenship, Matthew J. Cuneo, Dean A.A. Myles

Single Crystal Diffraction

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The Fenna-Matthews-Olson protein from Prosthecochloris aestuarii (PaFMO) has been crystallized in a new form that is amenable to high-resolution X-ray and neutron analysis. The crystals belonged to space group H3, with unit-cell parameters a = b = 83.64, c = 294.78Å, and diffracted X-rays to ∼1.7Å resolution at room temperature. Large PaFMO crystals grown to volumes of 0.3-0.5mm³ diffracted neutrons to 2.2Å resolution on the MaNDi neutron diffractometer at the Spallation Neutron Source. The resolution of the neutron data will allow direct determination of the positions of H atoms in the structure, which are believed to be fundamentally important in tuning the individual excitation energies of bacteriochlorophylls in this archetypal photosynthetic antenna complex. This is one of the largest unit-cell systems yet studied using neutron diffraction, and will allow the first high-resolution neutron analysis of a photosynthetic antenna complex.

Original language	English
Pages (from-to)	171-175
Number of pages	5
Journal	Acta Crystallographica Section F:Structural Biology Communications
Volume	75
DOIs	https://doi.org/10.1107/S2053230X19000724
State	Published - 2019

Keywords

Fenna-Matthews-Olson protein
Prosthecochloris aestuarii
hydrogen
neutron crystallography
neutron diffraction
photosynthesis
site energy

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10.1107/S2053230X19000724

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@article{ae58e4ed2406455f8edeb2df14bbd266,

title = "Neutron and X-ray analysis of the Fenna-Matthews-Olson photosynthetic antenna complex from Prosthecochloris aestuarii",

abstract = "The Fenna-Matthews-Olson protein from Prosthecochloris aestuarii (PaFMO) has been crystallized in a new form that is amenable to high-resolution X-ray and neutron analysis. The crystals belonged to space group H3, with unit-cell parameters a = b = 83.64, c = 294.78{\AA}, and diffracted X-rays to ∼1.7{\AA} resolution at room temperature. Large PaFMO crystals grown to volumes of 0.3-0.5mm3 diffracted neutrons to 2.2{\AA} resolution on the MaNDi neutron diffractometer at the Spallation Neutron Source. The resolution of the neutron data will allow direct determination of the positions of H atoms in the structure, which are believed to be fundamentally important in tuning the individual excitation energies of bacteriochlorophylls in this archetypal photosynthetic antenna complex. This is one of the largest unit-cell systems yet studied using neutron diffraction, and will allow the first high-resolution neutron analysis of a photosynthetic antenna complex.",

keywords = "Fenna-Matthews-Olson protein, Prosthecochloris aestuarii, hydrogen, neutron crystallography, neutron diffraction, photosynthesis, site energy",

author = "Xun Lu and Brinda Selvaraj and Sudipa Ghimire-Rijal and Orf, {Gregory S.} and Flora Meilleur and Blankenship, {Robert E.} and Cuneo, {Matthew J.} and Myles, {Dean A.A.}",

note = "Publisher Copyright: {\textcopyright} 2019 International Union of Crystallography.",

year = "2019",

doi = "10.1107/S2053230X19000724",

language = "English",

volume = "75",

pages = "171--175",

journal = "Acta Crystallographica Section F:Structural Biology Communications",

issn = "2053-230X",

publisher = "International Union of Crystallography",

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TY - JOUR

T1 - Neutron and X-ray analysis of the Fenna-Matthews-Olson photosynthetic antenna complex from Prosthecochloris aestuarii

AU - Lu, Xun

AU - Selvaraj, Brinda

AU - Ghimire-Rijal, Sudipa

AU - Orf, Gregory S.

AU - Meilleur, Flora

AU - Blankenship, Robert E.

AU - Cuneo, Matthew J.

AU - Myles, Dean A.A.

PY - 2019

Y1 - 2019

N2 - The Fenna-Matthews-Olson protein from Prosthecochloris aestuarii (PaFMO) has been crystallized in a new form that is amenable to high-resolution X-ray and neutron analysis. The crystals belonged to space group H3, with unit-cell parameters a = b = 83.64, c = 294.78Å, and diffracted X-rays to ∼1.7Å resolution at room temperature. Large PaFMO crystals grown to volumes of 0.3-0.5mm3 diffracted neutrons to 2.2Å resolution on the MaNDi neutron diffractometer at the Spallation Neutron Source. The resolution of the neutron data will allow direct determination of the positions of H atoms in the structure, which are believed to be fundamentally important in tuning the individual excitation energies of bacteriochlorophylls in this archetypal photosynthetic antenna complex. This is one of the largest unit-cell systems yet studied using neutron diffraction, and will allow the first high-resolution neutron analysis of a photosynthetic antenna complex.

AB - The Fenna-Matthews-Olson protein from Prosthecochloris aestuarii (PaFMO) has been crystallized in a new form that is amenable to high-resolution X-ray and neutron analysis. The crystals belonged to space group H3, with unit-cell parameters a = b = 83.64, c = 294.78Å, and diffracted X-rays to ∼1.7Å resolution at room temperature. Large PaFMO crystals grown to volumes of 0.3-0.5mm3 diffracted neutrons to 2.2Å resolution on the MaNDi neutron diffractometer at the Spallation Neutron Source. The resolution of the neutron data will allow direct determination of the positions of H atoms in the structure, which are believed to be fundamentally important in tuning the individual excitation energies of bacteriochlorophylls in this archetypal photosynthetic antenna complex. This is one of the largest unit-cell systems yet studied using neutron diffraction, and will allow the first high-resolution neutron analysis of a photosynthetic antenna complex.

KW - Fenna-Matthews-Olson protein

KW - Prosthecochloris aestuarii

KW - hydrogen

KW - neutron crystallography

KW - neutron diffraction

KW - photosynthesis

KW - site energy

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AN - SCOPUS:85062594171

SN - 2053-230X

VL - 75

SP - 171

EP - 175

JO - Acta Crystallographica Section F:Structural Biology Communications

JF - Acta Crystallographica Section F:Structural Biology Communications

ER -

Neutron and X-ray analysis of the Fenna-Matthews-Olson photosynthetic antenna complex from Prosthecochloris aestuarii

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