Nanoscopic lipid domains determined by microscopy and neutron scattering

Charles P. Collier; Dima Bolmatov; James G. Elkins; John Katsaras

doi:10.1016/j.ymeth.2024.01.020

Nanoscopic lipid domains determined by microscopy and neutron scattering

Charles P. Collier, Dima Bolmatov, James G. Elkins, John Katsaras

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Biological membranes are highly complex supramolecular assemblies, which play central roles in biology. However, their complexity makes them challenging to study their nanoscale structures. To overcome this challenge, model membranes assembled using reduced sets of membrane-associated biomolecules have been found to be both excellent and tractable proxies for biological membranes. Due to their relative simplicity, they have been studied using a range of biophysical characterization techniques. In this review article, we will briefly detail the use of fluorescence and electron microscopies, and X-ray and neutron scattering techniques used over the past few decades to study the nanostructure of biological membranes.

Original language	English
Pages (from-to)	127-135
Number of pages	9
Journal	Methods
Volume	223
DOIs	https://doi.org/10.1016/j.ymeth.2024.01.020
State	Published - Mar 2024

Funding

J.K. and C.P.C. are supported through the Scientific User Facilities Division of the Department of Energy (DOE) Office of Science , sponsored by the Basic Energy Science (BES) Program, DOE Office of Science, under Contract No. DE-AC05-00OR22725 . D.B. is supported through the National Science Foundation, Division of Molecular and Cellular Biosciences (MCB), under contract no. 2219289 .

Keywords

Cryo-EM
FRET
Lipid Bilayers
Model Membranes
SANS

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10.1016/j.ymeth.2024.01.020

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title = "Nanoscopic lipid domains determined by microscopy and neutron scattering",

abstract = "Biological membranes are highly complex supramolecular assemblies, which play central roles in biology. However, their complexity makes them challenging to study their nanoscale structures. To overcome this challenge, model membranes assembled using reduced sets of membrane-associated biomolecules have been found to be both excellent and tractable proxies for biological membranes. Due to their relative simplicity, they have been studied using a range of biophysical characterization techniques. In this review article, we will briefly detail the use of fluorescence and electron microscopies, and X-ray and neutron scattering techniques used over the past few decades to study the nanostructure of biological membranes.",

keywords = "Cryo-EM, FRET, Lipid Bilayers, Model Membranes, SANS",

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year = "2024",

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doi = "10.1016/j.ymeth.2024.01.020",

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T1 - Nanoscopic lipid domains determined by microscopy and neutron scattering

AU - Collier, Charles P.

AU - Bolmatov, Dima

AU - Elkins, James G.

AU - Katsaras, John

PY - 2024/3

Y1 - 2024/3

N2 - Biological membranes are highly complex supramolecular assemblies, which play central roles in biology. However, their complexity makes them challenging to study their nanoscale structures. To overcome this challenge, model membranes assembled using reduced sets of membrane-associated biomolecules have been found to be both excellent and tractable proxies for biological membranes. Due to their relative simplicity, they have been studied using a range of biophysical characterization techniques. In this review article, we will briefly detail the use of fluorescence and electron microscopies, and X-ray and neutron scattering techniques used over the past few decades to study the nanostructure of biological membranes.

AB - Biological membranes are highly complex supramolecular assemblies, which play central roles in biology. However, their complexity makes them challenging to study their nanoscale structures. To overcome this challenge, model membranes assembled using reduced sets of membrane-associated biomolecules have been found to be both excellent and tractable proxies for biological membranes. Due to their relative simplicity, they have been studied using a range of biophysical characterization techniques. In this review article, we will briefly detail the use of fluorescence and electron microscopies, and X-ray and neutron scattering techniques used over the past few decades to study the nanostructure of biological membranes.

KW - Cryo-EM

KW - FRET

KW - Lipid Bilayers

KW - Model Membranes

KW - SANS

UR - http://www.scopus.com/inward/record.url?scp=85185177834&partnerID=8YFLogxK

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DO - 10.1016/j.ymeth.2024.01.020

M3 - Article

C2 - 38331125

AN - SCOPUS:85185177834

SN - 1046-2023

VL - 223

SP - 127

EP - 135

JO - Methods

JF - Methods

ER -

Nanoscopic lipid domains determined by microscopy and neutron scattering

Abstract

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Keywords

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