Multi-enzyme logic network architectures for assessing injuries: Digital processing of biomarkers

Jan Halámek; Vera Bocharova; Soujanya Chinnapareddy; Joshua Ray Windmiller; Guinevere Strack; Min Chieh Chuang; Jian Zhou; Padmanabhan Santhosh; Gabriela V. Ramirez; Mary A. Arugula; Joseph Wang; Evgeny Katz

doi:10.1039/c0mb00153h

Multi-enzyme logic network architectures for assessing injuries: Digital processing of biomarkers

Jan Halámek
, Vera Bocharova
, Soujanya Chinnapareddy
, Joshua Ray Windmiller
, Guinevere Strack
, Min Chieh Chuang
, Jian Zhou
, Padmanabhan Santhosh
, Gabriela V. Ramirez
, Mary A. Arugula
, Joseph Wang
, Evgeny Katz

Research output: Contribution to journal › Article › peer-review

70 Scopus citations

Abstract

A multi-enzyme biocatalytic cascade processing simultaneously five biomarkers characteristic of traumatic brain injury (TBI) and soft tissue injury (STI) was developed. The system operates as a digital biosensor based on concerted function of 8 Boolean AND logic gates, resulting in the decision about the physiological conditions based on the logic analysis of complex patterns of the biomarkers. The system represents the first example of a multi-step/multi-enzyme biosensor with the built-in logic for the analysis of complex combinations of biochemical inputs. The approach is based on recent advances in enzyme-based biocomputing systems and the present paper demonstrates the potential applicability of biocomputing for developing novel digital biosensor networks.

Original language	English
Pages (from-to)	2554-2560
Number of pages	7
Journal	Molecular BioSystems
Volume	6
Issue number	12
DOIs	https://doi.org/10.1039/c0mb00153h
State	Published - Dec 2010
Externally published	Yes

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title = "Multi-enzyme logic network architectures for assessing injuries: Digital processing of biomarkers",

abstract = "A multi-enzyme biocatalytic cascade processing simultaneously five biomarkers characteristic of traumatic brain injury (TBI) and soft tissue injury (STI) was developed. The system operates as a digital biosensor based on concerted function of 8 Boolean AND logic gates, resulting in the decision about the physiological conditions based on the logic analysis of complex patterns of the biomarkers. The system represents the first example of a multi-step/multi-enzyme biosensor with the built-in logic for the analysis of complex combinations of biochemical inputs. The approach is based on recent advances in enzyme-based biocomputing systems and the present paper demonstrates the potential applicability of biocomputing for developing novel digital biosensor networks.",

author = "Jan Hal{\'a}mek and Vera Bocharova and Soujanya Chinnapareddy and Windmiller, \{Joshua Ray\} and Guinevere Strack and Chuang, \{Min Chieh\} and Jian Zhou and Padmanabhan Santhosh and Ramirez, \{Gabriela V.\} and Arugula, \{Mary A.\} and Joseph Wang and Evgeny Katz",

year = "2010",

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doi = "10.1039/c0mb00153h",

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T2 - Digital processing of biomarkers

AU - Halámek, Jan

AU - Bocharova, Vera

AU - Chinnapareddy, Soujanya

AU - Windmiller, Joshua Ray

AU - Strack, Guinevere

AU - Chuang, Min Chieh

AU - Zhou, Jian

AU - Santhosh, Padmanabhan

AU - Ramirez, Gabriela V.

AU - Arugula, Mary A.

AU - Wang, Joseph

AU - Katz, Evgeny

PY - 2010/12

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N2 - A multi-enzyme biocatalytic cascade processing simultaneously five biomarkers characteristic of traumatic brain injury (TBI) and soft tissue injury (STI) was developed. The system operates as a digital biosensor based on concerted function of 8 Boolean AND logic gates, resulting in the decision about the physiological conditions based on the logic analysis of complex patterns of the biomarkers. The system represents the first example of a multi-step/multi-enzyme biosensor with the built-in logic for the analysis of complex combinations of biochemical inputs. The approach is based on recent advances in enzyme-based biocomputing systems and the present paper demonstrates the potential applicability of biocomputing for developing novel digital biosensor networks.

AB - A multi-enzyme biocatalytic cascade processing simultaneously five biomarkers characteristic of traumatic brain injury (TBI) and soft tissue injury (STI) was developed. The system operates as a digital biosensor based on concerted function of 8 Boolean AND logic gates, resulting in the decision about the physiological conditions based on the logic analysis of complex patterns of the biomarkers. The system represents the first example of a multi-step/multi-enzyme biosensor with the built-in logic for the analysis of complex combinations of biochemical inputs. The approach is based on recent advances in enzyme-based biocomputing systems and the present paper demonstrates the potential applicability of biocomputing for developing novel digital biosensor networks.

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DO - 10.1039/c0mb00153h

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SN - 1742-206X

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JO - Molecular BioSystems

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IS - 12

ER -

Multi-enzyme logic network architectures for assessing injuries: Digital processing of biomarkers

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