Multi-enzyme logic network architectures for assessing injuries: Digital processing of biomarkers

Jan Halámek; Vera Bocharova; Soujanya Chinnapareddy; Joshua Ray Windmiller; Guinevere Strack; Min Chieh Chuang; Jian Zhou; Padmanabhan Santhosh; Gabriela V. Ramirez; Mary A. Arugula; Joseph Wang; Evgeny Katz

doi:10.1039/c0mb00153h

Multi-enzyme logic network architectures for assessing injuries: Digital processing of biomarkers

Jan Halámek, Vera Bocharova, Soujanya Chinnapareddy, Joshua Ray Windmiller, Guinevere Strack, Min Chieh Chuang, Jian Zhou, Padmanabhan Santhosh, Gabriela V. Ramirez, Mary A. Arugula, Joseph Wang, Evgeny Katz

Research output: Contribution to journal › Article › peer-review

70 Scopus citations

Abstract

A multi-enzyme biocatalytic cascade processing simultaneously five biomarkers characteristic of traumatic brain injury (TBI) and soft tissue injury (STI) was developed. The system operates as a digital biosensor based on concerted function of 8 Boolean AND logic gates, resulting in the decision about the physiological conditions based on the logic analysis of complex patterns of the biomarkers. The system represents the first example of a multi-step/multi-enzyme biosensor with the built-in logic for the analysis of complex combinations of biochemical inputs. The approach is based on recent advances in enzyme-based biocomputing systems and the present paper demonstrates the potential applicability of biocomputing for developing novel digital biosensor networks.

Original language	English
Pages (from-to)	2554-2560
Number of pages	7
Journal	Molecular BioSystems
Volume	6
Issue number	12
DOIs	https://doi.org/10.1039/c0mb00153h
State	Published - Dec 2010
Externally published	Yes

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title = "Multi-enzyme logic network architectures for assessing injuries: Digital processing of biomarkers",

abstract = "A multi-enzyme biocatalytic cascade processing simultaneously five biomarkers characteristic of traumatic brain injury (TBI) and soft tissue injury (STI) was developed. The system operates as a digital biosensor based on concerted function of 8 Boolean AND logic gates, resulting in the decision about the physiological conditions based on the logic analysis of complex patterns of the biomarkers. The system represents the first example of a multi-step/multi-enzyme biosensor with the built-in logic for the analysis of complex combinations of biochemical inputs. The approach is based on recent advances in enzyme-based biocomputing systems and the present paper demonstrates the potential applicability of biocomputing for developing novel digital biosensor networks.",

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AU - Halámek, Jan

AU - Bocharova, Vera

AU - Chinnapareddy, Soujanya

AU - Windmiller, Joshua Ray

AU - Strack, Guinevere

AU - Chuang, Min Chieh

AU - Zhou, Jian

AU - Santhosh, Padmanabhan

AU - Ramirez, Gabriela V.

AU - Arugula, Mary A.

AU - Wang, Joseph

AU - Katz, Evgeny

PY - 2010/12

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AB - A multi-enzyme biocatalytic cascade processing simultaneously five biomarkers characteristic of traumatic brain injury (TBI) and soft tissue injury (STI) was developed. The system operates as a digital biosensor based on concerted function of 8 Boolean AND logic gates, resulting in the decision about the physiological conditions based on the logic analysis of complex patterns of the biomarkers. The system represents the first example of a multi-step/multi-enzyme biosensor with the built-in logic for the analysis of complex combinations of biochemical inputs. The approach is based on recent advances in enzyme-based biocomputing systems and the present paper demonstrates the potential applicability of biocomputing for developing novel digital biosensor networks.

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Multi-enzyme logic network architectures for assessing injuries: Digital processing of biomarkers

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